针刺“足三里”穴对脾虚证模型大鼠肠系膜淋巴结T淋巴细胞亚群的影响

Effect of acupuncture at Zusanli on T lymphocyte subsets in mesenteric lymph nodes of spleen deficiency syndrome rats

背景:肠道及相关淋巴组织是构成肠道免疫的重要组成部分,在以脾虚水液代谢障碍为主要消化道症状中肠道免疫起到了重要的调节作用。目的:观察针刺对脾虚症大鼠淋巴结组织T淋巴细胞亚群的影响,探讨脾虚与肠道黏膜免疫之间相关性及针刺治疗脾虚证的作用机制。方法:选用36只雌性SD大鼠随机分为3组,模型组,针刺组,空白组。通过劳倦伤脾加饮食失节多因素复合法建立脾虚大鼠模型,造模31 d;造模成功后针刺组针刺大鼠双侧"足三里"穴;模型组自然恢复;空白组不予干预。造模期间及治疗期间检测各组大鼠尿D-木糖排泄率检测,治疗结束后,摘取小肠肠系膜淋巴结,免疫组织化学法观察大鼠肠系膜淋巴结T淋巴细胞亚群的变化。结果与结论:(1)在脾虚状态下模型组、针刺组相比空白组大鼠尿D-木糖排泄率明显降低(P<0.05或P<0.01),针刺治疗后针刺组尿D-木糖排泄率与模型组相比明显升高(P<0.01),稍低于空白组(P<0.05);(2)模型组、针刺组CD4+T淋巴细胞计数均低于空白组(P<0.01);针刺组CD4+T巴细胞计数高于模型组(P<0.01);(3)模型组CD8+T淋巴细胞计数明显低于空白组(P<0.01);针刺组CD8+T淋巴细胞计略数低于空白组(P<0.05),高于模型组(P<0.01);(4)模型组和针刺组CD4+/CD8+T淋巴细胞比值明显低于空白组(P<0.05),针刺组高于模型组(P<0.01);(5)结果表明,针刺"足三里"穴能够提高脾虚证大鼠尿D-木糖排泄率,提高小肠的消化吸收能力,明显改善改善脾虚证大鼠纳差、便溏、泄泻等消化系统症状;调节脾虚证大鼠肠系膜淋巴结T淋巴细胞亚群的平衡,提高其肠道免疫功能,调整肠道消化功能紊乱状态,维持机体免疫耐受,调节机体免疫稳态。

BACKGROUND:Intestinal and lymphoid tissues constitute an important part of intestinal immunity,which plays an important regulatory role in spleen deficiency and hydronephrosis.OBJECTIVE:To observe the effect of acupuncture on T lymphocyte subsets in lymph nodes of rats with spleen deficiency,and to investigate the correlation of spleen deficiency with intestinal immunity and the mechanism of acupuncture for spleen deficiency syndrome.METHODS:Thirty-six female Sprague-Dawley rats were randomly divided into three groups:model,acupuncture and blank control groups.The rat model of spleen deficiency was established by fatigue-induced spleen injury plus abnormal diet for 31 days.Afterwards,the rats in the acupuncture group received acupuncture at Zusanli(ST 36).Urine D-xylose excretion rate was detected during modeling and treatment.Then,the mesenteric lymph nodes were removed,and the changes in T lymphocyte subsets in the mesentericlymph nodes were observed by immunohistochemistry.RESULTS AND CONCLUSION:Urine D-xylose excretion rate under spleen deficiency in the modeling and acupuncture groups was significantly lower than that in the blank control group(P<0.05 or P<0.01);after acupuncture,the urine D-xylose excretion rate was significantly increased compared with the modeling group(P<0.01),but still lower than that in the blank control group(P<0.05).The count of CD4+T lymphocytes,count of CD8+T lymphocytes and ratio of CD4+/CD8+T lymphocytes were ranked as follows:blank control group>acupuncture group>modeling group(P<0.01 or P<0.05).These results suggest that acupuncture at Zusanli can improve the urine D-xylose excretion rate,regulate the balance of T lymphocyte subsets in mesenteric lymph node of rats with spleen deficiency,thus improving the intestinal immune function,spleen deficiency systems,disorder of intestinal digestive function,intestinal digestion and absorption,as well as anorexia,loose stool,diarrhea and other symptoms of the digestive system.