人尿源性干细胞的分离培养及生物学特性

Isolation, culture and biological characteristics of human urine-derived stem cells

背景:尿源性干细胞作为干细胞家族新兴的群体,在组织工程构建方面越来越引起人们的重视。目的:研究尿源性干细胞的培养方法,对尿源性干细胞进行生物学鉴定。方法:取健康成人清洁尿液,将其离心、分离、培养、传代获取尿源性干细胞,用MTT法检测细胞的生长曲线,流式细胞仪检测细胞表面标志物,并对细胞进行成骨、成脂诱导能力鉴定。结果与结论:(1)从尿液中分离获取得到生长迅速并大量增殖的尿源性干细胞,对该细胞进行培养传代,细胞生长曲线成"S"型;(2)尿源性干细胞表达间充质干细胞表面标志物CD29、CD44、CD73、CD90,保持了干细胞的活性;(3)尿源性干细胞有向成骨、成脂细胞分化的生物学特性;(4)结果表明,尿源性干细胞有很强的增殖分化能力,作为一种经济便利无创的细胞来源,为以后研究尿道缺损、器官再造等提供巨大的便利。

BACKGROUND:As an emerging stem cell family,urine-derived stem cells have attracted more and more attentions in the tissue engineering construction.OBJECTIVE:To study the culture method of urine-derived stem cells,and to identify the biological characteristics of urine-derived stem cells.METHODS:Cleaning urine samples were harvested and centrifuged,and urine-derived stem cells were isolated from the urine samples and extensively expanded in vitro.Cell growth curve was measured by MTT method,and cell surface markers were detected by flow cytometry.Meanwhile,the cells were subjected to osteogenic and adipogenic induction.RESULTS AND CONCLUSION:Urine-derived stem cells were successfully isolated from the urine samples,which grew and proliferated rapidly.After subculture,the cells exhibited an S-shaped growth.The isolated urine-derived stem cells expressed CD29,CD44,CD73 and CD90,indicating that the cells maintained the activity of stem cells.Moreover,the isolated cells had the ability of osteogenic and adipogenic differentiation.To conclude,urine-derived stem cells have strong proliferation and differentiation potentials,as a kind of economic,convenient,non-invasive source of cells,which can provide great convenience for urethral defect repair and organ reconstruction.