受胆固醇调节的变异剪接体及其意义

Cholesterol-regulated Variant Spliceosomes and Their Significance

目的:检测人肝癌细胞中LDLR的变异剪接体LDLR-?Exon4、LDLR-?Exon12,HMGCS1的变异剪接体HMGCS1-?Exon2和HMGCR的变异剪接体HMGCR-Exon?13是否受胆固醇的调节。方法:建立高胆固醇模型组、低胆固醇模型组及正常组,提取不同模型组的RNA并进行反转录,采用Realtime PCR(RT-PCR)技术检测LDLR-?Exon4,LDLR-?Exon12,HMGCS1-?Exon2和HMGCR-Exon?13的mRNA的表达。结果:在高胆固醇模型组中,与正常组相比,LDLR-?Exon4、LDLR-?Exon12、HMGCS1-?Exon2及HMGCR-Exon?13的mRNA的表达量与各基因全长相比均明显升高(P<0.05);而在低胆固醇模型组中,与正常组相比,上述的变异剪接体mRNA的表达量与各基因全长相比均明显下降(P<0.05)。结论:LDLR-?Exon4、LDLR-?Exon12、HMGCS1-?Exon2及HMGCR-Exon?13等变异剪接体受胆固醇的调节,并可能参与并调控胆固醇的代谢。

Objective:To detect whether LDLR,HMGCS1 and HMGCR alternatively spliced variants LDLR-ΔExon4,LDLR-ΔExon12,HMGCS1-ΔExon2 and HMGCR-ΔExon13 regulated by cholesterol in human hepatocellular carcinoma cells.Method:The high cholesterol model group and the low cholesterol model group were established,and compared with the normal group.RNA extraction of different model cells was reverse transcribed,and Real-time PCR(RT-PCR)technology was used to detect mRNA expression of LDLR-ΔExon4,LDLR-ΔExon12,HMGCS1-ΔExon2 and HMGCR-ΔExon13.Result:Compared with the normal group,mRNA expression of LDLR-ΔExon4,LDLR-ΔExon12,HMGCS1-ΔExon2 and HMGCR-ΔExon13 were increased in the high cholesterol model group(P<0.05).However,mRNA expression of LDLR-ΔExon4,LDLR-ΔExon12,HMGCS1-ΔExon2 and HMGCR-ΔExon13 were decreased in the low cholesterol model group compared with the normal group(P<0.05).Conclusion:LDLR-ΔExon4,LDLR-ΔExon12,HMGCS1-ΔExon2 and HMGCR-ΔExon13 spliced variants are regulated by cholesterol and may participate in regulating the metabolism of cholesterol.