卷烟加工及储存过程中细菌群落组成及变化研究

Effects of Cigarette Processing and Storage Process on Bacterial Community Structure

[目的]研究卷烟加工及储存过程中细菌群落结构以及多样性的变化。[方法]采用二代高通量测序平台,以云烟(软珍,R)不同制丝工艺的加工工序及储存过程的烟叶表面微生物为研究对象,对其进行16S rRNA基因高通量测序。[结果]尽管不同制丝工艺在相同加工工序中微生物群落丰富度不尽相同,但主要高丰富度微生物类群的种类比较一致;此外,烟叶在不同城市的不同储存时间下,微生物类群的变化具有类似的趋势。[结论]该研究对从微生物角度改进烟叶制丝工艺具有一定的借鉴意义,同时表明烟叶的储存时间可能是影响微生物后期群落发生变化的主要因素。

[Objective]In order to study the changes of bacterial community structure and diversity in cigarette processing and storage process[Method]The two generation high-throughput sequencing platforms were used to study the processing technology of Yunyan tobacco(R)and the surface microorganism of tobacco leaves during storage.The 16S rRNA gene was sequenced by high-throughput sequencing.[Result]Although different technics in the same processing in the process of microbial community richness was not the same,but the main types of high richness microbial communities were consistent.In addition,the results also showed that the storage time of tobacco leaves could be the main factor affecting the changing of microbial community rather than the cities where they storage.[Conclusion]The study has a certain reference significance for improving tobacco processing technology from microorganism angle.Meanwhile,it indicates that the storage time of tobacco leaves may be the main factor that affects the later stage of microbial community.