拟南芥SGR2基因参与叶绿素降解和衰老过程

Arabidopsis thaliana SGR2 Genes Involved in Degradation and Senescence of Chlorophyll

[目的]研究拟南芥SGR2基因参与叶绿素降解和衰老过程。[方法]购得SGR2(stay green rice,水稻滞绿基因)基因的T-DNA插入突变体,并进行叶绿素荧光分析、叶绿素含量和可溶性总糖测定、qRT-PCR等试验以及生物信息学分析。[结果]突变体叶绿素a含量较WT(colo-0野生型)降低;可溶性糖含量高于WT;RBCS(Rubisco,核酮糖1,5-二磷酸羧化酶/加氧酶的小亚基)和CAB(Chl a binding protein,叶绿素a结合蛋白)基因表达下调。而突变体开花明显比野生型延迟。[结论]证明了SGR2基因参与了拟南芥叶绿素降解和衰老过程。

[Objective]To study Arabidopsis thaliana SGR2 genes involved in degradation and senescence of chlorophyll.[Method]T-DNA insertion mutants for SGR2 gene were got.The experiments of chlorophyll fluorescence analysis,determination of the content of chlorophyll and soluble sugar,qRT-PCR test and some other bioinformatics analysis were conducted.[Result]The chlorophyll content of the mutant leaves was less than the case in the wild-type.The soluble sugar content of the mutant leaves was more than the case in the wild-type.Expression of RBCS and CAB were down regulated.While,a transparently prolonged flowering period was observed in mutant,as compared with colo-0.[Conclusion]The results demonstrate that SGR2 gene is involved degradation and senescence of chlorophyll in A.thaliana.