大豆疫霉菌PsSed5编码基因转录分析及功能研究

Transcriptional Analysis and Functional Study of PsSed5 Gene of Phytopnthora sojae

[目的]研究大豆疫霉菌(Phytophthora sojae)SNARE家族之一的编码基因Ps Sed5转录及功能。[方法]利用实时定量PCR分析Ps Sed5在大豆疫霉不同生活史及侵染时间段的转录,利用PEG介导的原生质体稳定转化法,将该基因沉默后分析沉默转化子的表型。[结果]Ps Sed5是组成性表达,在侵染早期表达量较高,Ps Sed5的2个沉默转化子菌丝生长减慢,致病力减弱。[结论]Ps Sed5在大豆疫霉的生长发育及致病性方面都发挥着重要的调控作用。

[Objective]To analyze the transcription and the gene function of PsSed5,one of the SNARE proteins in Phytophthora sojae.[Method]Using the real-time PCR analyzed the PsSed5 expression in different developmental and infection stages.Using the PEG-mediated DNA transformation obtained the PsSed5 silencing transformants to research its phenotype.[Result]The PsSed5 is constitutively expressed during various life stages and the course of infection,with the relatively highest expression levels in early infection,while the hypha from the silencing transformants growth slowly with weak pathogenicity.[Conclusion]The results show that PsSed5 is important for growth and contributes to the full virulence of P.sojae.