抗戊唑醇苹果轮纹病菌株的渗透压敏感性及其膜透性研究

Study on the Osmolarity Sensitivity and Membrane Permeability of Tebuconazole-resistant Strains of Botryosphaeria dothidea

本文通过比较苹果轮纹病菌低、中、高抗戊唑醇菌株与敏感菌株的渗透压敏感性及其膜透性的差异,探讨苹果轮纹病菌抗戊唑醇菌株的生理生化变化趋势。结果显示:在不同浓度葡萄糖和NaCl的渗透压条件下,敏、抗菌株均表现为先升高再下降的趋势,且敏感、高抗菌株的菌落直径一直小于低、中抗菌株,对渗透压的变化也更敏感。葡萄糖浓度在5~80 mg/L时,所有菌株菌落直径缓慢增加;在80~150 mg/L时,中抗菌株菌落直径开始减小,其他菌株则急剧升高后下降,且敏、高抗菌株的菌落菌丝稀疏,生长势较弱。NaCl浓度在0~10 g/L时,所有菌株的菌落直径缓慢增加;浓度>10 g/L时,菌落直径急剧减小,在80 g/L时菌落直径最小,且在相同处理条件下表现为低抗菌株>中抗菌株>敏感菌株>高抗菌株的趋势。不同浓度戊唑醇处理,敏、抗菌株的相对电导率变化趋势一致:在10~20min急速上升,>20 min后相对电导率变化幅度减小,呈缓慢上升趋势;但高、中抗菌株的相对电导率一直高于敏、低抗菌株,敏感菌株的相对电导率最低,说明高、中抗菌株膜透性比敏感菌株大。上述结果将为进一步探讨苹果轮纹病菌对戊唑醇的抗性机制,为指导生产的科学用药提供理论依据。

By comparing the differences of osmotic sensitivity and membrane permeability in low,medium and high tebuconazole resistant strains and sensitive strains of Botryosphaeria dothidea,the trend of physiological and biochemical changes of its tebuconazole resistant strains were discussed.The result showed that,under the osmotic pressure of different concentrations of glucose and NaCl,sensitive and antibacterial plants showed a trend of increasing first and then decreasing.The bacterial colony diameter of sensitive and high resistant strains has been smaller than that of low and medium strains,and is more sensitive to the changes of osmotic pressure.When the concentration of glucose was 5～80 mg/L,the colony diameter of all strains increased slowly.In the 80～150 mg/L,the colony diameter of the antimicrobial strains began to decrease,and the other strains increased sharply and then decreased.The colony hypha of sensitive and the high-resistance anti-bacterial strain was sparse,and the growth potential was weak.When the NaCl concentration was 0～10 g/L,the colony diameter of all strains increased slowly,when NaCl concentration was>10g/L,the colony diameter decreased sharply.At 80 g/L the colony diameter was the smallest.Under the same treatment,there was a tendency of low antibacterial strain>medium antibacterial strain>sensitive strain>high resistant strain.Under the conditions of different concentrations of tebuconazole,the relative conductivity change trend of the sensitive and antibacterial strain was consistent.The relative conductivity increased rapidly at 10～20 min and decreased after>20 min,showing a slow rising trend.The relative conductivity of the medium and high antibacterial strains was always higher than that of the sensitive and low antimicrobial strains,and the relative conductivity of the sensitive strains was the lowest,indicating that the membrane permeability of the high and medium antibacterial strains was higher than that of the sensitive strains.The above results will further explore the resistance mechanism of Botryosphaeria dothidea and provide theoretical basis for the production of scientific medicine.