支原体肺炎患儿的机体炎症反应、Th1/Th2免疫应答评估及其与病原菌负荷的相关性

The inflammatory response and Th1/Th2 immune response in children with mycoplasma pneumonia and their correlation with pathogen load

目的:研究支原体肺炎患儿的机体炎症反应、Th1/Th2免疫应答评估及其与病原菌负荷的相关性。方法:选择在本院诊断为肺炎支原体肺炎的120例患儿并根据诱导痰中肺炎支原体DNA的拷贝数分为低负荷组和高负荷组,另取同期在志丹县人民医院健康体检的健康儿童作为对照组。检测血清中炎症反应细胞因子、Th1/Th2细胞因子的含量以及外周血中Th1/Th2转录因子的表达量。结果:低负荷组和高负荷组患儿血清中sTREM-1、CRP、sICAM-1、PCT、TNF-α、IFN-γ、IL-2、IL-4、IL-10的含量以及外周血中T-bet、GATA3的mRNA表达量均显著高于对照组,外周血中SOCS1、SOCS3的mRNA表达量均显著低于对照组;高负荷组患儿血清中sTREM-1、CRP、sICAM-1、PCT、TNF-α、IFN-γ、IL-2、IL-4、IL-10的含量以及外周血中T-bet、GATA3的mRNA表达量均显著高于低负荷组,外周血中SOCS1、SOCS3的mRNA表达量均显著低于低负荷组。结论:支原体肺炎患儿体内病原菌负荷的增加会造成炎症反应和Th1/Th2免疫应答过度激活。

Objective:To study the inflammatory response and Th1/Th2 immune response in children with mycoplasma pneumonia and their correlation with pathogen load.Methods:120 children who were diagnosed with mycoplasma pneumoniae pneumonia in Zhidan County People's Hospital between March 2014 and March 2017 were selected and divided into low load group and high load group according to the mycoplasma pneumoniae DNA copy number in the induced sputum,and healthy children who received physical examination in Zhidan County People's Hospital during the same period were selected as control group.The contents of inflammatory response cytokines and Th1/Th2 cytokines in serum as well as the expression of Th1/Th2 transcription factors in peripheral blood were detected.Results:Serum sTREM 1,CRP,sICAM 1,PCT,TNFα,IFNγ,IL 2,IL 4 and IL 10 levels as well as peripheral blood T bet and GATA3 mRNA expression of low load group and high load group were significantly higher than those of control group while peripheral blood SOCS1 and SOCS3 mRNA expression were significantly lower than those of control group;serum sTREM 1,CRP,sICAM 1,PCT,TNFα,IFNγ,IL 2,IL 4 and IL 10 levels as well as peripheral blood T bet and GATA3 mRNA expression of high load group were significantly higher than those of low load group while peripheral blood SOCS1 and SOCS3 mRNA expression were significantly lower than those of low load group.Conclusion:The increase of pathogen load in children with mycoplasma pneumoniae can cause the overactivation of inflammatory response and Th1/Th2 immune response.