阿萨希毛孢子菌通过分泌铁载体在低铁限制性条件下生长

Trichosporon asahii could growunder iron-restricted condition through siderophore secretion

目的探讨阿萨希毛孢子菌在低铁限制性条件下生长的机制,为后续联合常用抗真菌药物治疗播散性毛孢子菌病提供实验室依据。方法 200μmol/L的去铁胺加入酵母浸出粉胨葡萄糖培养基(Yeast Extract Peptone Dextrose Medium,YPD)培养基中制备低铁限制性培养基,检测阿萨希毛孢子菌临床分离株在该培养基中的生长曲线,并检测低铁条件下,阿萨希毛孢子菌产生铁载体的情况。结果阿萨希毛孢子菌的生长曲线不受低铁限制性条件的影响,且其在低铁环境下可以产生铁载体。结论阿萨希毛孢子菌通过产生铁载体在低铁限制性条件下正常生长。

Objective To investigate the mechanism of the growth of Trichosporon asahii under iron-restricted condition,and provide laboratory evidence for the combined therapy with antifungals against disseminated trichosporonosis.Methods 200μmol/L BPS was added to the YPD medium to prepare iron-restricted condition,then the growth curve of Trichosporon asahii in this medium was detected.Results The Trichosporon asahii grew normally under the iron-restricted condition,and siderophore was detected from the culture medium supernatants simultaneously.Conclusions Trichosporon asahii grew normally under the iron-restricted condition through siderophore secretion.