西红康治疗早期糖尿病肾病的实验研究

Experimental Study on Xihongkang in Treatment of Early Diabetic Nephropathy

目的:探讨西红康治疗早期糖尿病肾病(diabetic nephropathy,DN)的实验研究。方法:62只大鼠中选取50只进行肾切除并一次性腹腔注射50 mg/kg链脲佐菌素(STZ)复制DN大鼠模型,48只造模成功并随机分为模型组、西红康组、开博通组、糖适平组,其余不做任何处理的为正常对照组,每组各12只,连续给药4周,检测并记录大鼠血糖、24 h尿蛋白、肾重/体重、尿素氮(BUN)、肌酐(Scr)、糖化血红蛋白(Hb A1c)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、脂联素及E-选择素含量。结果:模型组24 h尿蛋白、肾重/体重、Hb A1c、Scr、BUN、血糖、TC、TG、LDL-C、脂联素及E-选择素含量均较正常对照组升高(P<0.01),HDL-C含量较正常对照组降低(P<0.01);西红康组、开博通组、糖适平组的24 h尿蛋白、肾重/体重、Hb A1c、Scr、BUN、血糖、TC、TG、LDL-C、脂联素及E-选择素含量均较模型组降低(P<0.01),HDL-C含量较模型组升高(P<0.01)。结论:西红康能够通过降低DN大鼠尿蛋白、血糖及血脂水平,减少脂联素分泌及E-选择素表达,进而延缓DN大鼠发展。

Objective:To explore experimental study on xihongkang in treatment of early diabetic nephropathy(diabetic nephropathy,DN).Method:In 62 rats,50 rats were selected for nephrectomy and intraperitoneal injection of 50 mg/kg streptozotocin(STZ)to replicate the DN rat model,48 rats were randomly divided into model group,xihongkang group,captopril group,gliguidone group,the other without any treatment as normal control group,12 rats in each group,administered continuously for 4 weeks.The concentration of blood glucose,24 h urine protein,kidney weight/body weight,blood urea nitrogen(BUN),creatinine(Scr),glycosylated hemoglobin(HbA1c),total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),adiponectin and E-selectin was detected and recorded.Result:The contents of 24 h urine protein,kidney weight/body weight,HbA1c,Scr,BUN,blood glucose,TC,TG,LDL-C,adiponectin and E-selectin of model group were higher than those of normal control group(P<0.01),and HDL-C content was lower than that of normal control group(P<0.01).The contents of 24 h urine protein,kidney weight/body weight,HbA1c,Scr,BUN,blood glucose,TC,TG,LDL-C,adiponectin and E-selectin of xihongkang group,captopril group,gliguidone group were lower than those of model group(P<0.01),and HDL-C content was higher than that of model group(P<0.01).Conclusion:Xihongkang can reduce the DN rat urine protein,blood glucose and blood lipid levels,reduced adiponectin secretion and expression of E-selectin,and delay the development of DN rats.