五味子醇甲对糖尿病ED大鼠勃起功能的影响

Effect of Schisandrin on the erectile function of diabetic ED rats

目的研究五味子醇甲对糖尿病勃起功能障碍(erectile dysfunction,ED)大鼠勃起功能的影响。方法100只雄性SD大鼠注射链脲佐菌素,构建糖尿病大鼠模型。利用阿朴吗啡诱导阴茎勃起,筛选出糖尿病ED大鼠。经过8周的五味子醇甲处理后(分低、中、高剂量组),测定各组大鼠海绵体内压(intracavernous pressure,ICP)和平均动脉压(mean arterial pressure,MAP),经下腔静脉取血测定丙氨酸转氨酶(alanine aminotransferse,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)、血清肌酐(serum creatinine,SCr)水平。结果电刺激前,各组间基础ICP差异无统计学意义(P>0.05)。电刺激时,各组间MAP差异无统计学意义(P>0.05);电刺激时,与正常对照组相比,糖尿病对照组、糖尿病性勃起功能障碍(diabetes-induced erection dysfunction,DIED)组大鼠ICP及ICP/MAP依次递减(P<0.05),与DIED组相比,低剂量药物组ICP及ICP/MAP无明显改变(P>0.05),中、高剂量药物组ICP及ICP/MAP依次递增(P<0.05),高剂量药物组上升幅度最大。各组间ALT、AST、ALP及SCr水平差异均无统计学意义(P>0.05)。结论五味子醇甲干预后可以改善DIED大鼠勃起功能,且安全性好,为今后该药物临床应用提供了理论依据。

Objective To study the effect of Schisandrin on the erectile function of the diabetic rats with erectile dysfunction(ED).Methods One handred healthy male SD rats were injected with streptozocin to establish the diabetic rat models.Apomorphine inducing penile erection was used to select the diabetic rats with ED.After 8 weeks of treatment with Schisandrin(low-dose group,middle-dose group and high-dose group),the intracavernous pressure(ICP)and mean arterial blood pressure(MAP)were measured.The inferior vena cava blood of rats were collected to measure the level of alanine aminotransferse(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)and serum creatinine(SCr).Results The baseline ICP among all groups have no obvious difference before the electrical stimulation(P>0.05).The MAP among all groups have no obvious difference when the electrical stimulation(P>0.05).When the electrical stimulation,compared with normal control group,the ICP and ICP/MAP of rats from diabetes mellitus and diabetes-induced erection dysfunction(DIED)groups were jointly decreased in sequence(P<0.05).Compared with the DIED group,the ICP and ICP/MAP of rats from DIED and low-dose Schisandrin group had no significant changes(P>0.05),and the ICP and ICP/MAP of rats from middle-dose Schisandrin group and high-dose Schisandrin group were both increased in sequence(P<0.05).The increase in the high-dose group was most obvious(P<0.05).There was no obvious difference among all groups on the levels of ALT,AST,ALP and SCr(P>0.05).Conclusion The application of Schisandrin is able to improve the erection function of rats with DIED and its safety is high.It provides theoretical basis for the clinical application of this drug in the future.