产蛋白酶、淀粉酶芽孢杆菌的筛选及鉴定

Screening and Identification of Bacillus Producing Protease and Amylase

采用酪素平板和可溶性淀粉平板初筛,产酶活力测定复筛,获得一株具有产蛋白酶、淀粉酶复合酶芽胞杆菌菌株CA02003。经酶活力测定,CA02003菌株在36 h时蛋白酶、淀粉酶均达到酶活最高峰,酶活力分别为8 723.16、251.19 U·m L^(-1)。通过形态特征、生理生化特征以及gyr B基因进化分析结果,鉴定为地衣芽孢杆菌。

The strain CA02003 which produce protease and amylase was screened by using soluble starch plate and casein plate,and rescreening it by determining the activity of enzyme production.By enzyme activity determina-tion,protease and amylase of CA02003 strain reached the highest peak of enzyme activity at 36 h,and the enzyme ac-tivity was 8723.16 and 251.19 U·mL-1 respectively.Through the colony morphology,physiological,biochemical char-acteristics and gyrB gene phylogenetic analysis,strain CA02003 was identified as Bacillus licheniformis.