噻吩取代长链查尔酮对肿瘤侵袭的抑制作用

Tumor invasion inhibition of thiophene substituted long chain chalcones

目的阐明一类新型长链查耳酮化合物与肿瘤侵袭的构效关系。方法以延长骨架提升特异性为基本理念,采用噻吩作为羰基端的主要衍生基团,以TSAHC为先导化合物,得到6个新查耳酮类化合物。采用Transwell法评估所得化合物的抗肿瘤侵袭作用,并对关键蛋白MMP-2进行评估,最后采用SYBYL软件对这6个新化合物进行分子对接。结果所得的6个化合物的结构经H-NMR和MS确认,其中化合物2、3显现出较好的抑制肿瘤迁移能力。对接结果显示,该类化合物中的磺酰基与噻吩基团对于化合物与靶点的结合有正贡献。结论细胞实验和分子对接共同显示,以噻吩为衍生基团的长链化修饰可以有效提高该类化合物抗肿瘤迁移的能力。

Aim To elucidate the structure-activity relationship between a new class of long chain chalcone compounds and tumor invasion.Methods The basic idea of the research was to enhance the specificity by prolonging the molecular structure.Based on the lead compound TSAHC,the thiophene was used as the main derivative at the carbonyl groups to obtain six new chalcones.Then we evaluated the anti-tumor activities of the compounds and the expression of key protein MMP-2 of the tumor invasion.Finally,six new compounds were docked to the protein by the SYBYL software.Results The structures of the six compounds were confirmed by H-NMR and MS.Among them,compound 2,3 showed fine capability to inhibit tumor invasion.The docking results also showed that the sulfonamide and thiophene groups of the compounds had positive contribution to the target binding of the compounds.Conclusion Cell experiments and molecular docking show that the long chain modification of chalcone by using thiophene as a derivative group can significantly enhance the anti-tumor invasion.