阿司匹林对HER-2阳性乳腺癌细胞SKBR3侵袭和迁移能力的影响

Effect of aspirin on invasion and migration of HER-2-positive breast cancer SKBR3 cells

目的探讨阿司匹林对乳腺癌细胞SKBR3侵袭和迁移能力的影响。方法先采用MTT法检测不同浓度(2.5、5.0、10.0、20.0、40.0 mmol/L)阿司匹林及DMSO(对照组)对SKBR3细胞生长抑制的影响。然后,将乳腺癌细胞SKBR3分为3组,即2.5 mmol/L阿司匹林组、10.0 mmol/L阿司匹林组和对照组(DMSO处理),采用Transwell实验、划痕实验分别检测细胞的侵袭和迁移能力,实验重复3次。Transwell实验和划痕实验结果数据比较,采用单因素方差分析,组间两两比较采用LSD法。结果 MTT结果显示,随着阿司匹林浓度的增大,SKBR3细胞生长抑制明显增加,其半数抑制浓度(IC_(50))为7.91 mmol/L。Transwell实验显示,对照组侵袭细胞染色570 nm吸光度值为2.232±0.054,2.5 mmol/L组为1.648±0.069,10.0 mmol/L组为0.372±0.019,3组间相比,细胞侵袭能力的差异有统计学意义(F=338.1,P<0.001);并且,与对照组相比,2.5 mmol/L和10.0 mmol/L阿司匹林组细胞侵袭能力明显受到抑制(P均<0.001)。划痕实验显示,24 h后对照组细胞迁移愈合率为(69.78±2.87)%,2.5 mmol/L阿司匹林组为(50.16±3.10)%,10.0 mmol/L阿司匹林组为(16.08±2.10)%,3组间相比,细胞迁移能力的差异也有统计学意义(F=100.8,P<0.001);并且,与对照组相比,2.5 mmol/L和10.0 mmol/L阿司匹林组细胞迁移能力均明显受到抑制(P均<0.050)。结论阿司匹林能够抑制乳腺癌细胞SKBR3的侵袭和迁移能力。

Objective To investigate the effect of aspirin on the invasion and migration of breast cancer SKBR3 cells.MethodsMTT assay was used to measure the inhibitory effect of aspirin(2.5、5.0、10.0、20.0、40.0 mmol/L)and DMSO(as control)on growth of SKBR3 cells.SKBR3 cells were cultured and divided into three groups,treated by 2.5 mmol/L,10.0 mmol/L aspirin and DMSO(as control)respectively.Transwell migration assay and scratch assay were used to measure the invasion and migration ability of cells.All experiments were repeated three times.The results in the Transwell migration assay and scratch assay were analyzed by one-way ANOVA and pairwise comparison was conducted by LSD method.ResultsMTT results showed that with the increase of aspirin concentration,growth inhibition of SKBR3 cells was increased significantly,with the half maximal inhibitory concentration(IC50)value of 7.91 mmol/L.Transwell migration assay showed that the optical density at the wavelength of 570 nm was 2.232±0.054 in control group,1.648±0.069 in 2.5 mmol/L aspirin group and 0.372±0.019 in 10.0 mmol/L aspirin group,respectively,indicating a significant difference in the invasion ability among groups(F=338.1,P<0.001).Compared with control group,the invasion ability was significantly inhibited in 2.5 mmol/L and 10.0 mmol/L aspirin groups(both P<0.001).The scratch assay showed that the migration healing rate at 24 h was(69.78±2.87)%in control group,(50.16±3.10)%in 2.5 mmol/L aspirin group and(16.08±2.10)%in 10.0 mg/L aspirin group,suggesting a significant difference in migration ability among groups(F=100.8,P<0.001).Compared with control group,the migration ability of cells in 2.5 mmol/L and 10.0 mmol/L aspirin groups was significantly inhibited,respectively(both P<0.050).ConclusionAspirin can inhibit the invasion and migration ability of breast cancer SKBR3 cells.