柠檬香茅叶水提液对黄秋葵的化感效应及其成分分析

Allelopathic Inhibition on Hibiscus esculentus and Chemical Composition of Aqueous Extract from Cymbopogon citratus Leaves

为研究柠檬香茅Cymbopogon citratus叶片对黄秋葵Hibiscus esculentus的化感作用,采用室内生物测定的方法,研究柠檬香茅叶水提液对黄秋葵种子萌发、幼苗生长的影响,并采用气质联用技术(GC-MS)分析柠檬香茅叶水提液的化学成分。结果表明:质量浓度为5g·L^(-1)的柠檬香茅叶水提液可促进黄秋葵种子发芽,质量浓度为30g·L^(-1)和40g·L^(-1)柠檬香茅叶水提液显著抑制黄秋葵种子萌发、根长生长、单株苗重,黄秋葵幼苗生长阶段对柠檬香茅叶水提液更敏感。通过鉴定得到柠檬香茅叶水提液中16种化合物,总含量占82.76%,相对含量较高的有香叶醛、橙花醛、香叶酸等,鉴定出的化合物中分别有酚酸类物质(8.27%)、脂肪酸类物质(2.12%)和萜类物质(52.19%)等已知的化感物质,相对含量总和达到62.58%。

Allelopathic effect of the aqueous extract from Cymbopogon citratus 1eaves on the germination and seedling growth of Hibiscus esculentus wasstudied in petri dish with laboratory bioassay.The chemical composition of the extract wasdetermined using GC-MS.Depending upon the concentration,the extractexhibited varied effects on the germination of H.esculentus seeds.At the concentration of 5 g·L-1,the extract stimulated the seed germination.In contrast,at 30 g·L-1 to 40 g·L-1 it displayed significant inhibitory effects on the germination,root growth as well asweight of individual seedlings.The seedlings were most sensitive to the extract during growth stage.There were 16 compounds identified by the analysis that represented 82.76%of the total content.Among them,geranial,nerol and geranic acid were the major constituents,which included phenolic acids(8.27%),fatty acids(2.12%)and terpenoids(52.19%)constituting 62.58%of the entirechemical composition in the extract.