呼出气体活体质谱分析揭示中医推拿引起的代谢反应

In vivo Breath Analysis by Extractive Electrospray Ionization-Mass Spectrometry for Investigation of Metabolic Responses to Traditional Chinese Medicine Massages

采用电喷雾萃取电离质谱(EESI-MS)技术,在正离子检测模式下,对经过临床中医诊断为肝胆火旺、心脾阳虚和寒湿厚重等不同健康状态的4名志愿者接受推拿前后的呼出气体样品在m/z 50~1000质量范围内的指纹谱图进行了直接分析。通过高分辨质谱确认了接受推拿前后发生显著变化的代谢物(如肾上腺素(m/z 184.0889)、3-(3-羟基苯基)丙酸(m/z 167.0615)和L-色氨酸(m/z 205.0933)等)的结构。此外,结合偏最小二乘法判别分析(PLS-DA)使健康状态不同的志愿者接受中医推拿前后呼出气体的指纹谱图得到明显区分,说明中医推拿能显著地改变志愿者的新陈代谢过程。呼出气体的直接质谱分析将是实时跟踪中医推拿引起的代谢变化的有效途径。

Traditional Chinese Medicine massage is a kind of physiotherapy which affects on specific parts of the body surface by means of training to regulate the function of the body to achieve the therapeutic effect.In this work,under positive detection model,the chemical fingerprint of exhaled breath from volunteers before and after receiving Traditional Chinese Medicine massage within m/z 50-1000 were detected by extractive electrospray ionization-mass spectrometry(EESI-MS).And through high resolution mass spectrometry analysis,the metabolites such as epinephrine(m/z 184.0889),3-(3-hydroxyphenyl)propionic acid(m/z 167.0615)and L-tryptophan(m/z 205.0933)were successfully identified.Besides,chemical fingerprints of volunteers before and after receiving Traditional Chinese Medicine massage under different health condition were clearly differentiated via partial least squares discrimination analysis(PLS-DA).The results showed that Traditional Chinese Medicine massage could significantly change the metabolic process of volunteers.Moreover,it further indicated that the established method could provide a real time fashion to follow metabolic changes caused by Traditional Chinese Medicine massage.