Cal27裸鼠口腔癌转移模型的建立及其意义

Establishment of Metastatic Model of Oral Cancer Using Cal27cell in Nude Mice and Its Significance

目的建立Cal27舌鳞癌动物正位移植瘤淋巴道转移模型,为进一步研究口腔癌细胞转移的规律及分子机制提供帮助.方法经口底将2×10~6 Cal27细胞悬液0.08 mL分别注射至24只裸鼠下颌舌骨肌与颏舌肌之间.成瘤后定期测量肿瘤体积,记录裸鼠体重变化;14 d后处死裸鼠,对组织切片型HE染色观察组织结构异型性和细胞异形性,颌下淋巴结及肺、肝有无转移,并统计转移率.结果裸鼠在接种Cal27细胞第3天成瘤,成瘤率100%(24/24).HE染色示肿瘤组织呈典型鳞癌表现.颌下淋巴结内可见转移鳞癌细胞,转移率70%(17/24).肝、肺未见转移.结论成功建立Cal27裸鼠正位移植瘤淋巴道转移模型.此动物模型能客观反应口腔癌生物学行为,模拟口腔癌区域淋巴结转移的过程,是研究口腔鳞状细胞癌侵袭转移较理想动物模型之一.

Objective To establish the orthotopic model of Cal27 tongue squamous carcinoma cells.Methods 2×106 Cal27 cells suspension was injected into floor of mouth of nude mice.Then the tumor volume was measured periodically and the body weight of the nude mice was recorded.14 days later,nude mice were killed,and the primary tumor and adjacent tissues,lungs,and liver were harvested.The tissue sections were stained with hematoxylin and eosin.The submandibular lymph nodes and lung,liver metastasis,and statistical metastasis rate were observed.Results The tumor formed after day 3 that nude mice were inoculated with Cal27 cells,and tumor formation rate was 100%(24/24).HE staining showed tumor tissue had typical performance of squamous carcinom.The squamous carcinoma cells were found in the submandibular lymph nodes,and the metastasis rate was 70%(17/24).There were no metastasis found in liver and lung.Conclusions Orthotopic metastatic model of oral cancer in nude mice has been established successfully.This animal model can objectively reflect the biological behavior of human oral cancer,is one of the ideal methods to study the invasion and metastasis of oral squamous cell carcinoma.