滋肾活血方对血管性痴呆大鼠NR2A表达的影响

Effect of Zishen Huoxue Decoction on Expression of NR2A in Rats with Vascular Dementia

目的探讨滋肾活血方对血管性痴呆(vascular dementia,VD)大鼠NR2A表达的影响。方法采用改良的双侧颈总动脉结扎法(2-VO法)制作VD大鼠模型,将其随机分为假手术组、模型组、滋肾活血组及奥拉西坦组。给药4周后,用免疫组化和RT-PCR检测NR2A及其m RNA的表达。结果与假手术组相比,模型组NR2A及其m RNA表达均降低(P<0.05);与模型组相比,滋肾活血组及奥拉西坦组NR2A及其m RNA表达增高(P<0.05)。结论滋肾活血方可提高VD大鼠的学习记忆能力,这可能与上调NR2A的表达有关。

Objective To investigate the effect of Zishen Huoxue decoction on the expressiom of NR2A in rats with vascular dementia(VD).Methods This VD model rats were built by the improved 2-VO.The rats were randomly divide into the sham-operation group,the model group,the Zishen Huoxue group and the oxiracetam group.After giving medicine for 4 weeks later,the expression of NR2A and its mRNA were measured by immunohistochemical analysis and RT-PCR.Results Compared with the model group,the expression of NR2A and its mRNA in Zishen Huoxu decoction group and oxiracetam group increased(P<0.05).Conclusion Zishen Huoxue decoction could improve the ability of learning and memory,which might be associated with the up-regulation of the expression of NR2A.