黄芪解毒汤对乳腺癌细胞Jag1基因和CyclinD1蛋白表达影响的研究

Effect of Huangqi Jiedu Decoction on Expression of Jag1 and CyclinD1 in Breast Cancer Cells

目的探讨黄芪解毒汤抑制乳腺癌复发转移的机制是否与其干预Jag1基因和CyclinD1蛋白在人乳腺癌MCF-7细胞中的表达有关。方法采用SPF级SD大鼠灌胃法制备黄芪解毒汤组、阿霉素组、参一胶囊组、生理盐水组的含药血清。运用细胞培养技术,MTT法检测发现最佳抑制浓度;以最佳抑菌浓度即20%浓度的含药血清干预人乳腺癌细胞,RT-PCR检测Jag1基因,Western-blot检测CyclinD1蛋白的表达。结果各组与生理盐水血清组比较,Jag1基因和CyclinD1蛋白表达均明显降低(P<0.01);与其他各组比较阿霉素血清组抑制作用最强(P<0.01);黄芪解毒汤血清组与参一胶囊血清组比较差异无统计学意义(P>0.05)。结论黄芪解毒汤可显著降低MCF-7细胞Jag1基因和CyclinD1蛋白表达,该结果可为黄芪解毒汤在乳腺癌术后治疗中的作用提供实验依据。

Objective To investigate the effect of Huangqi Jiedu decoction on the expression of Jag1 gene and CyclinD1 protein in MCF-7 cells of human breast cancer and to reveal its possible mechanism of inhibiting the recurrence and metastasis of breast cancer.Methods Drug-containing serum of Huangqi Jiedu decoction group,doxorubicin group,Shenyi capsule group and normal saline serum group were built by using the method of gavage in SPF SD rats.The optimal concentration of cell inhibition routine culture of cells was detected by MTT.The breast cancer was intervened with 20%drug containing serum.The expression of JAG1 protein was detected by real-time quantitative PCR and CyclinD1 protein was determined by Western-blot.Results Compared with the normal saline group,the expression of Jag1 and CyclinD1 protein in the other serum groups significantly decreased(P<0.01).The inhibitory effect of the adriamycin serum group was the strongest(P<0.01).There was no significant difference between the serum group of Huangqi Jiedu decoction group and Shenyi capsule group(P>0.05).Conclusion Huangqi Jiedu decoction could significantly prohibit the expression of Jag1 and CyclinD1,and provide the experimental evidence for Huangqi Jiedu Decoction in the treatment of breast cancer after surgery.