新疆地方梨品种S基因型的鉴定

Identification of S-genotypes of Native Pear Cultivars in Xinjiang

【目的】鉴定与研究9个新疆地方梨品种S基因型,为库尔勒香梨授粉品种的筛选及其遗传改良提供理论依据。【方法】设计特异性引物对9个新疆地方梨品种的基因组DNA进行PCR扩增,片段回收、克隆、测序,使用生物信息学软件对各序列进行分析。【结果】褐色句句梨、轮台句句梨、霍城冬黄梨、八月梨、库尔勒黄酸梨、句句梨、奎克阿木特1号等7个品种的S基因型分别是:S28S4、S28S4、S28S1、S28S4、S22S35、S28S4、S36S34S22;可特阿木特和棋盘梨各鉴定出一条基因,S基因型分别是S8Sx和S28Sx。【结论】确定了7个新疆地方梨品种S基因型,可特阿木特和棋盘梨中各有1条S基因没有被鉴别出。丰富了我国梨资源的S基因型信息,为库尔勒香梨的丰产栽培和遗传改良提供了依据。

【Objective】To provide theoretical basis for the selection of pollinated varieties and genetic improvement of Korla pear by classifying and studying the S genotypes of 9 native pear varieties in Xinjiang.【Method】A specific primer was designed to amplify the genomic DNA of 9 native pear varieties in Xinjiang,including PCR amplification,fragment recovery,cloning,sequencing,and the analysis of each sequence by bioinformatics software.【Result】The S-genotypes of the seven cultivars were identified as follows:Hesejujuli S28S4,Luntaijujuli S28S4,Hesejujuli:S28S4,Luntaijujuli:S28S4,Huochengdonghuangli:S28S1,Bayueli:S28S4,Kuerlehuangsuanli:S22S35,Jujuli:S28S4,Kuikeamute1hao:S36S34S22.One gene was identified as S8Sx and another gene as S28Sx in Keteamute Pear and Qipanli Pear,respectively.【Conclusion】It has enriched the s-genotypes information of pear cultivars in China and provided a certain guidance gist for high yield cultivation and genetic improvement of Korla pear.