转Bt Cry1Ac基因抗虫棉花外源基因漂移研究

Study on Exogenous Gene Flow from Transgenic Bt Cry1Ac Cotton

【目的】研究转Bt Cry1Ac基因棉花外源基因漂移规律,为转基因棉花生态安全性评价提供依据。【方法】2014年在吐鲁番市和2015年在库尔勒市,利用小区试验种植转基因棉花,翌年利用卡那霉素抗性检测结合分子检测的方法检测外源基因的漂移。【结果】在距离转基因棉花小区边缘1~120 m均能检测到含有外源基因Cry1Ac。SGK321种子漂移率最大漂移率是向南方向距离转基因棉花小区边缘1 m处,漂移率为5%;GK19最大漂移率是向西方向达到了10.09%。以方向因素和距离因素建立Cry1Ac基因漂移Logistic回归预测模型,方向和距离对种子漂移都有显著影响(P<0.05),但方向和距离两个因子并不能最终决定外源基因的漂移率。【结论】转基因棉花外源基因漂移率受方向和距离的影响,但最终决定外源基因的漂移率,并不是方向和距离联合作用的结果。

【Objective】To clarify the extraneous gene drift rule of BT Cry1Ac transgenic cotton and provide the basis for ecological safety evaluation of transgenic cotton,we carried out this study in Turpan and Korla for two years.【Method】Transgenic cotton was planted in the plot experiment,and the drift distance of exogenous gene was detected by using Kanamycin resistance detection combined with molecular detection the following year.【Result】These results showed that either the source of SGK321 or GK19,the exogenous gene Cry1Ac could be detected from 1-120 m.The maximum drift rate of SGK321 seed drift rate was 1 m from the edge of the transgenic cotton area in the south direction,and the drift rate was 5%.GK19 drift rate was biggest to the west up to 10.09%.So,based on direction and distance factors,Cry1Ac gene drift Logistic regression forecast model was built,and these results showed that the direction and distance of seed drift had a significant effect(P<0.05),however,direction and distance could not ultimately determine the extraneous gene drift rate.【Conclusion】The extraneous gene drift rate of transgenic cotton was affected by direction and distance,but it was not the result of the combination of direction and distance that ultimately determined the extraneous gene drift rate.