加速溶剂萃取-QuEChERS法测定高活性干酵母粉中12种有机持久污染物残留

Determination of 12 Persistent Organic Pollutants in Highly Active Dry Yeast Powder by Accelerated Solvent Extraction-QuEChERS

建立了加速溶剂萃取(ASE)-QuEChERS-气相色谱-质谱法测定高活性干酵母粉中六氯苯、α-666、七氯等12种有机持久污染物(POPs)的分析方法。样品经乙腈加速溶剂萃取,N-丙基乙二胺(PSA)净化,气相色谱-质谱法选择离子监测模式(GC-MS-SIM)检测。结果表明,12种POPs在0.025~2.500μg/m L范围内线性关系良好,相关系数R2≥0.992,在3个添加水平(0.05、0.25、1.00 mg/kg)的平均回收率在74.5%~111.7%之间,相对标准偏差(RSDs)为3.1%~11.3%(n=6),检出限(LODs)为0.002~0.009 mg/kg。本方法操作简便、快速、节省溶剂,能够满足高活性干酵母粉中12种POPs残留检测需求。

A method for the simultaneous determination of 12 persistent organic pollutants(POPs)for example hexachlorobenzene,α-666,heptachlor,etc in highly active dry yeast powder was developed using accelerated solvent extraction(ASE)-QuEChERS-gas chromatography-mass.The Sample was extracted by acetonitrile with accelerated solvent extraction apparatus,and then cleaned up by PSA.The qualification and quantification of target compound were performed by gas chromatography-mass in selected ion monitoring mode(GC-MS-SIM).The results show that the 12 POPs were linear in the range of 0.025~2.500μg/mL with good correlation coefficients(R2≥0.992).The average recoveries of the 12 POPs in highly active dry yeast powder were 74.5%~111.7%with relative standard deviations(RSDs,n=6)of 3.1%~11.3%,at the spiked level of 0.05,0.25,1.00 mg/kg.The limits of detection of the method(LODs)were 0.002~0.009 mg/kg.The method is convenient operation,rapid,saving on solvent,could meet determination of 12 POPs residual in highly active dry yeast powder.