黑肩绿盲蝽对水稻挥发物单一组分的嗅觉行为反应

The Olfactory Behavior Response of Cytorhinus lividipennis to Single Component of Rice Volatiles

研究水稻挥发物单一组分对稻飞虱(Nilaparvata lugens)天敌黑肩绿盲蝽(Cyrtorhinus lividipennis)嗅觉行为反应的影响,为进一步开发基于水稻挥发物的害虫天敌引诱剂提供一定的理论依据。利用T型嗅觉仪室内测试了黑肩绿盲蝽对10种水稻挥发物单一组分(苯乙醛、2-庚酮、α-蒎烯、芳樟醇、反-2-己烯醛、叶醇、罗勒烯、水杨酸甲酯、橙花叔醇、石竹烯)不同浓度(100、10、1、0.1 mg·L^(-1))的嗅觉行为反应。试验结果表明,α-蒎烯(100 mg·L^(-1))、水杨酸甲酯(10 mg·L^(-1))、芳樟醇(1 mg·L^(-1))、罗勒烯(1 mg·L^(-1))、反-2-己烯醛(0.1、1、10、100 mg·L^(-1))、叶醇(0.1 mg·L^(-1))等6种化合物对黑肩绿盲蝽雌成虫具有引诱效果,而芳樟醇(10、100 mg·L^(-1))、叶醇(100 mg·L^(-1))、橙花叔醇(1、10、100 mg·L^(-1))、β-石竹烯(0.1、1、10、100 mg·L^(-1))对其产生了驱避效果。同种化合物在不同浓度下的作用效果不同。黑肩绿盲蝽会对水稻挥发性化合物单一组分产生一定的嗅觉行为反应,且在不同水稻挥发物不同浓度下的选择行为存在一定的差异。水稻挥发物单一组分的浓度对黑肩绿盲蝽的嗅觉选择行为具有一定的影响,如芳樟醇和叶醇在低浓度下表现出引诱而高浓度下表现出趋避;反-2-己烯醛随浓度的增加引诱效果减弱,而橙花叔醇随浓度的增加驱趋避效果增强。

To identify the chemical signals that attract rice planthopper predators Cytorhinus lividipennis Reuter and thereby provide basic information for further study of rice volatile agents,the olfactory response of Cytorhinus lividipennis to 10 kinds of single rice volatile compounds(benzeneacetaldehyde,2-heptanone,α-pinene,methyl salicylate,linalool,(E)-2-hexenal,ocimene,cis-3-Hexen-1-ol,β-caryophyllene,nerolidol)in 4 different concentrations(100,10,1,0.1 mg·L-1)was tested by T shaped olfactometer.The results showed that Cytorhinus lividipennis was attracted byα-pinene(100 mg·L-1),methyl salicylate(10 mg·L-1),linalool(1 mg·L-1),ocimene(1 mg·L-1),(E)-2-hexenal(0.1,1,10,100 mg·L-1)and cis-3-hexen-1-ol(0.1 mg·L-1),but was repelled by linalool(10,100 mg·L-1),cis-3-hexen-1-ol(100 mg·L-1),nerolidol(1,10,100 mg·L-1),β-caryophyllene(0.1,1,10,100 mg·L-1).Besides,the olfactory response was related to the concentrations.The olfactory response showed that Cytorhinus lividipennis was impacted by rice volatile compounds,but it was different between types of rice volatiles and concentrations.The concentration of single component of rice volatiles would impact the olfactory selection behavior,such as linalool and cis-3-hexen-1-ol would attract predators at low concentration but repelled predators at high concentration.The attraction effect of(E)-2-hexenal was decreased with the increase of concentration,but the repellent effect of nerolidol was enhanced with the increase of concentration.The results of this experiment would be useful in practical applications of the chemicals to control pest and utilize natural enemies in the field.