三七总皂苷对过氧化氢诱导HT22细胞氧化损伤的保护作用

Protective effect of panax notoginseng saponins on oxidative injury induced by hydrogen peroxide in HT22 cells

目的研究三七总皂苷(PNS)对氧化氢H_2O_2损伤后海马神经元HT22细胞的保护作用。方法采用CCK-8法检测作用PNSHT22细胞后细胞生长活性的变化,并通H_2O_2刺激HT22细胞,观察PNS对HT22细胞活性的影响及形态变化。采用乳酸脱氢酶检测细胞损伤情况,流式细胞仪检测细胞的凋亡比例。结果PNS在有效浓度范围内对HT22细胞无毒性,且能增加细胞活性,减轻H_2O_2对其损伤程度。结论PNS能通过增强细胞内抗氧化活性,提高HT22细胞的存活率,从而保护细胞抗氧化损伤。

Objective To study the protective effect of panax notoginseng saponins(PNS)on hippocampal neurons HT22 cells after injury by hydrogen peroxide(H2O2).Methods CCK-8 was used to detect the cell growth activity of HT22 cells after the action of PNS.After stimulation with H2O2,the effect of PNS on the activity and morphological changes of HT22 cells were observed.Cell damage was checked using LDH test.Flow cytometry was used to count the percentage of apoptotic cells.Results Panax notoginseng saponins had no toxic effect on HT22 cells in effective concentration range,and could enhance the cell vitality,significantly reduce the cell damage caused by H2O2.Conclusions Panax notoginseng saponins can enhance the intracellular antioxidant activity to enhance the survival rate of HT22 cells,thus protect the cells from oxidative damage.