低温处理对继代多次马铃薯组培苗复壮程度的影响

Effect of Low Temperature Treatment on Rejuvenation Degree of Subculture Seedlings of Potato

为给继代多次的马铃薯组培苗复壮提供理论依据,定量分析了低温处理对马铃薯组培瓶苗强壮程度、光合作用能力、整体代谢水平及成活率的影响。结果表明,9℃低温处理40 d,可使低温处理代苗的地径、叶绿素含量增加,并最终提高了生根培养代苗的地径、叶绿素含量、可溶性蛋白含量以及成活率。说明低温处理可对继代多次的马铃薯组培苗起到良好的复壮作用,对出瓶苗的成活率和代谢水平有较大的提高效应。

To provide theoretical basis for the rejuvenation of potato tissue culture seedlings,the influence of low temperature treatment on the strength,photosynthesis ability,overall metabolism level and survival rate of potato tissue culture bottle seedlings were analyzed quantitatively.The results showed that the ground diameter and chlorophyll content of the seedlings increased at 9℃for 40 d.Finally,the ground diameter,chlorophyll content,soluble protein content and survival rate of the rooting culture generations increased.In a word,the low temperature treatment can play a good rejuvenation effect on the potato tissue culture seedlings.The survival rate and metabolism level of the bottle seedlings are greatly improved.