羟基氧化钴长余辉纳米探针用于食品中VC的检测

Detection of Vitamin C in Food Base on Cobalt Oxyhydroxide Persistent Luminescent (CoOOH-PLNP) Nanaoparticles

采用水热两相法结合高温短时煅烧,制备出一种具有超高亮度、单分散尺寸及光学稳定近红外发光的三掺杂镓锗酸锌长余辉纳米材料(ZGGO∶Cr^(3+),Yb^(3+),Er^(3+)persistent luminescence nanoparticles,ZGGO∶Cr^(3+),Yb^(3+),Er^(3+)PLNPs),在其表面修饰羟基氧化钴(Co OOH),并通过Co OOH与VC之间的特异性氧化还原反应构建出一种检测VC的纳米探针。该探针以ZGGO∶Cr^(3+),Yb^(3+),Er^(3+)PLNPs作为光学信号单元,Co OOH为猝灭剂,基于荧光内滤效应,实现对VC高选择性、高灵敏度的快速检测。结果表明,Co OOH可有效猝灭PLNPs的发光;VC可通过还原Co OOH使PLNPs的发光得到恢复,且荧光恢复与VC含量具有良好的线性相关性(R2=0.993 15),检出限为1.18 mg/kg。用于实际样品猕猴桃、柠檬、草莓中VC的检测,测定结果与高效液相色谱的检测结果具有较好的一致性。此外,合成的ZGGO∶Cr^(3+),Yb^(3+),Er^(3+)PLNPs在近红外区具有超长的余辉性能,测定时无需激发,可进一步用于细胞及活体内VC的监测与成像。

Vitamin C is a small water-soluble molecule in vivo which can be directly involved in the redox reaction,with a variety of physiological activities.Herein,an ultra-bright triple-doped zinc gallogermanate nanostructure with favorable monodispersity and optically stable near infrared(NIR)luminescence whose surface was modified with cobalt oxyhydroxide(CoOOH)was synthesized by a surfactant-aided hydrothermal method in combination with short time calcination,and a nanoprobe for the detection of vitamin C was built based on the specific reaction of CoOOH and vitamin C.The nanoprobe used the ZGGO:Cr3+,Yb3+,Er3+persistent luminescence nanoparticles(PLNPs)as optical signal units and CoOOH as quencher,allowing the detection of vitamin C with high selectivity and sensitivity based on the inner filter effect(IFE).The results showed that CoOOH could effectively quench the luminescence of PLNPs.Vitamin C could restore the luminescence of PLNPs by reducing CoOOH,which displayed a good linear correlation with the content of vitamin C(R2=0.993 15)and a detection limit(LOD)of 1.18 mg/kg.When applied to real samples of lemon,strawberry and kiwi fruit,this method gave results consistent with those from HPLC.In addition,the synthesized ZGGO:Cr3+,Yb3+,Er3+PLNPs had a long afterglow performance in the near infrared(NIR)region,and therefore could be applied to real-time monitoring and imaging of vitamin C in living cells and organisms without in situ excitation.