高血压患者RYR-3基因多态性与大动脉粥样硬化性卒中的相关性研究

Correlation between RYR-3 Gene Polymorphism and Large Artery Atherosclerosis-Related Stroke in Patients with Hypertension

目的:探讨中国北方汉族人群中,高血压患者兰尼碱受体-3(RYR-3)基因多态性与大动脉粥样硬化性卒中的关系。方法:选择高血压合并大动脉粥样硬化性卒中患者232例为卒中组,高血压非卒中且不伴有颈动脉狭窄的患者200例为高血压组,非高血压健康体检者200例为对照组,采用基质辅助激光解吸电离飞行时间质谱法比较RYR-3基因型多态性在各组间的差异。结果:卒中组RYR-3基因rs877087位点基因型和等位基因与高血压组比较,差异均无统计学意义(均P>0.05);卒中组和对照组的基因型和等位基因的差异均有统计学意义(均P<0.05)。卒中组RYR-3基因rs2229116位点基因型和等位基因与高血压组比较,差异均有统计学意义(均P<0.05)。多因素Logistic回归分析结果显示RYR-3基因rs2229116基因型AA是高血压患者发生LAA的危险因素(OR=1.155,95%CI 1.018-1.626,P=0.030)。结论:对于高血压患者,RYR-3基因rs2229116位点多态性与大动脉粥样硬化性卒中密切相关。

Objective:To ivestigate tie genetic association between the ryanodie receptor-3(RYR-polymorphism of patients with hypertension and large a-ery atherosclerosis(LAA)in the Han descent popula tion in Northern Chma.Metliods:Total 232 patients experiencmg hypertension and LAA-related stroke were recruited to participate rn the study as the stroke group.Two hundred hyperpietic patients without ce-ical artery stenosis were recruited as the hypertension group.Two hundred healthy physical examination patients without hypertension from our hospital physical examiiation centre were selected as the control goup.Genotype was determined by matrix-assisted laser desorptionionization time of flight mass spectrometry for the polymorphism the RYR-3 gene.The genotype frequencies of the RYR-3 gene among groups were analyzed.Results:There was no significant difference i the frequencies of genotype and allele of RYR-3 gene rs877087 between the stroke group and the hypertension goup(both P>0.05).However,there was a significant difference between the stroke group and the control group(both P<0.05).There was a significant difference i the frequencies of geno-tyye and allele of RYR-3 gene rs2229116 between the stroke goup and the hypertension group(both P>0.05).For patients with hypertension,multivariate logistic regression analysis showed that polymorphism of RYR-3 gene rs2229116(OR=1.155,95$CI 1.018-1.626,P=0.030)was a risk factor for LAA-related stroke.Conclusion:In patients with hypertension,polymorphism of RYR-3 gene rs2229116 may be closely liked to large artery atherosclerosis-related stroke.