摘要
目的:探索人参皂苷Rb1对糖尿病大鼠肝脏糖脂代谢的影响及其调控机制。方法:链脲佐菌素(Streptozotocin,STZ)处理建立糖尿病大鼠模型。HE染色观察肝脏病变。TUNEL染色检测肝细胞凋亡情况。血糖仪检测血糖浓度。放射免疫分析检测胰岛素和C肽水平。运用全自动化分析仪测定总胆固醇、三酰甘油、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇浓度。蛋白印迹检测JNK信号通路相关蛋白JNK1、c-Jun表达。免疫组化分析炎症因子IL-6、IL-1β和TNF-α蛋白表达。结果:人参皂苷Rb1可改善糖尿病大鼠肝损伤。与健康对照组相比,STZ诱导组的血糖浓度明显升高,胰岛素及C肽浓度明显降低(P<0.05)。与STZ诱导组相比,STZ+人参皂苷Rb1组的血糖浓度明显降低,胰岛素及C肽浓度明显升高(P<0.05)。与健康对照组相比,STZ诱导组的总胆固醇、三酰甘油、低密度脂蛋白胆固醇升高,高密度脂蛋白胆固醇降低(P<0.05)。与STZ诱导组相比,STZ+人参皂苷Rb1组总胆固醇、三酰甘油、低密度脂蛋白胆固醇降低,高密度脂蛋白胆固醇升高(P<0.05)。STZ诱导组JNK1和c-Jun蛋白相对表达量明显高于健康对照组(P<0.05)。STZ+人参皂苷Rb1组JNK1和c-Jun蛋白相对表达量明显低于STZ诱导组(P<0.05)。与健康对照组相比,STZ诱导组的IL-6、IL-1β和TNF-α表达明显升高。STZ+人参皂苷Rb1组IL-6、IL-1β和TNF-α表达明显低于STZ诱导组。结论:人参皂苷Rb1可通过JNK信号通路改善糖尿病大鼠肝损伤及糖脂代谢异常,降低炎症反应。
Objective:To explore effects and mechanism on glucolipid metabolism of liver of ginsenoside Rb1 in diabetic rats.Methods:Diabetic rat model was induced by streptozotocin(STZ).Hepatic pathological changes were observed by hematoxylin-eosin(HE)staining.Apoptosis was analyzed through Terminal deoxynucleotidyl transferase-mediated dUTP nick labeling(TUNEL)staining.Blood glucose was determined with glucometer.The level of insulin and C peptide was tested by radioimmunoassay.Total cholesterol,low density lipoprotein cholesterol,high-density lipoprotein cholesterol,triglyceride was detected by full automatic biochemical analyzer.The expression of JNK signal pathway related proteins JNK1 and c-Jun was measured by Western blot.The expression of inflammatory factor IL-6,IL-1βand TNF-αwas detected by immunohistochemistry.Results:Liver injury was ameliorated by ginsenoside Rb1 in diabetic rats.Compared with control group,the level of blood glucose in STZ-induced diabetic rat group was increased with attenuated level of insulin and C peptide(P<0.05).Compared with STZ-induced diabetic rat group,the level of blood glucose in STZ+ginsenoside Rb1 group was decreased with enhancive level of insulin and C peptide(P<0.05).Compared with control group,the level of total cholesterol,triglyceride and low density lipoprotein cholesterol in STZ-induced diabetic rat group was elevated with attenuated level of high-density lipoprotein cholesterol(P<0.05).Compared with STZ-induced diabetic rat group,the level of total cholesterol,triglyceride and low density lipoprotein cholesterol in STZ+ginsenoside Rb1 group declined with elevated level of high-density lipoprotein cholesterol(P<0.05).The relative expression of JNK1 and c-Jun in STZ-induced diabetic rat group was higher than control group(P<0.05).The relative expression of JNK1 and c-Jun in STZ+ginsenoside Rb1 group was lower than STZ-induced diabetic rat group(P<0.05).Compared with control group,the expression of IL-6,IL-1βand TNF-αin STZ-induced diabetic rat group was enhanced(P<0.05).The expression of IL-6,IL-1βand TNF-αin STZ+ginsenoside Rb1 group was lower than STZ-induced diabetic rat group(P<0.05).Conclusion:Ginsenoside Rb1 ameliorates liver injury,abnormal glucolipid metabolism and inflammatory response of liver through inhibition of JNK signal pathway in diabetic rats.
作者
曹萌
CAO Meng(The Second Department of Endocrinology,First Affiliated Hospital of Xinxiang Medical College,Xinxiang 453100,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2018年第4期531-536,548,共7页
Chinese Journal of Immunology
基金
2016-2017年度河南省省教育厅课题(No.16A320043)