急性呼吸道感染儿童9种呼吸道病原体IgM抗体检测分析及其与空气污染的相关性探讨

Relationship between detection rate of antibodies of 9 types of atypical respiratory pathogens IgM in children with acute respiratory tract infection and air pollutants

目的:了解并探讨急性呼吸道感染儿童9种呼吸道病原体Ig M抗体检出情况及其与空气污染的相关性。方法:实时记录天津市2015年6月～2017年5月空气质量检测数据,并收集同期于天津市儿童医院住院的急性呼吸道感染患儿的血清,采用间接免疫荧光法检测9种呼吸道病原体,了解其检出情况并分析其与空气污染物之间的相关性。结果:共检测33 078例患儿,其中病原体阳性者共有8 364例,病原体检出总阳性率为25.29%,包括腺病毒(ADV)152例(0.46%)、Q热立克次体(COX)9例(0.03%)、肺炎衣原体(CPN)14例(0.04%)、甲型流感病毒(FLu A)3例(0.01%)、乙型流感病毒(FLu B)930例(2.8%)、嗜肺军团菌(LP)96例(0.29%)、肺炎支原体(MP)6 719例(20.3%)、副流感病毒(PIV)326例(0.99%)、呼吸道合胞病毒(RSV)115例(0.35%)。MP和FLu B为最主要病原体。天津市调查期间PM2.5、PM10、NO_2的平均浓度值均处于较高水平;PM2.5浓度最高,PM10次之。AQI、PM2.5、PM10、NO_2、SO_2浓度与住院病例数均具有相关性,PM10.0、NO_2、SO_2浓度与病原体总的阳性例数具有相关性,SO_2浓度与MP阳性病例关系最为密切。结论:天津市儿童医院2015年6月～2017年5月就诊患儿9种呼吸道病原体检出阳性率以MP最高,其次是Flu B。天津市空气污染严重,AQI、空气污染物浓度与住院病例数以及病原体总的阳性例数都有关,SO_2浓度与MP阳性病例关系密切。

Objective:To understand and explore the detection rate of the antibodies of 9 types of a typical respiratory pathogens IgM in children with acute respiratory tract infection and the relationship with air pollutants.Methods:The data of air quality monitoring in Tianjin from June 2015 to May 2017 were recorded,and the serum of hospitalized children with ARI were collected during the same period.Detection the antibodies of 9 types of atypical respiratory pathogens IgM by method of indirect immunofluorescence.To understand the detection rate of the antibodies of 9 types of atypical respiratory pathogens IgM and explore the relationship with air pollutants.Results:Among the 33 078 samples,8 364 samples were positive and total positive rate was 25.29%.ADV accounted for 152(0.46%)in all these samples,COX accounted for 9(0.03%),CPN accounted for 14(0.04%),FLuA accounted for 3(0.01%),FLuB accounted for 930(2.8%),LP accounted for 96(0.29%),MP accounted for 6 719(20.3%),PIV accounted for 326(0.99%),RSV accounted for 115(0.35%).The MP and FLuB were major pathogens.During the survey,the average PM2.5,PM10 and NO 2 were all at a high level,with PM2.5 being the highest and PM10 being the second highest.The AQI and the concentration of PM2.5,PM10,NO 2 and SO 2 had correlation with hospitalized cases,the concentration of PM10,NO 2,SO 2 had correlation with and the total positive samples,and the correlation between the concentration of SO 2 and MP was the closest.Conclusion:The positive rate of MP was the hightest in hospitalized children with ARI of Tianjin children′s hospital from June 2015 to May 2017,followed by FluB.Tianjin has severe air pollution,AQI and air pollutants has correlation with the number of hospitalized patients and the total positive samples,correlation between the concentration of SO 2 and MP was the closest.