DSA彩色编码技术在评估兔骨骼肌缺血再灌注损伤中的应用

Application of parametric DSA color coding on skeletal muscle ischemia reperfusion injury in rabbit model

目的:利用DSA彩色编码成像技术(syngo iFlow)观察兔后肢急性缺血再灌注后肌肉组织的iFlow参数变化,探讨其在评价骨骼肌缺血再灌注损伤中的应用价值。方法:新西兰大白兔30只平均随机分成缺血再灌注(ischemia reperfusion,IR)0 h、6 h、12 h、24 h组与假手术对照组。IR各组分别在右下肢缺血3 h后再灌注0 h、6 h、12 h、24 h行双下肢动脉造影,利用syngo iFlow软件分析造影图像并得到iFlow参数(rPeak)。同时检测各组血清中CK、LDH、MDA、SOD的含量,比较各组的血清生化指标及rPeak的差异,并分析rPeak与生化指标的相关性。结果:与对照组比较,IR各组的血清CK、LDH及MDA值升高,SOD值降低(P均<0.05);且随着再灌注时间的延长,血清LDH及CK含量逐渐升高。而再灌注各组间的MDA、SOD含量差异无统计学意义。IR各组的rPeak值均低于对照组,且随着IR时间的延长逐渐下降(P<0.05)。rPeak与LDH、CK、MDA呈负相关(r分别为-0.885、-0.908、-0.541,P均<0.05),与SOD呈正相关(r=0.832,P<0.05)。结论:iFlow参数rPeak与实验室指标有较好的相关性,能够在一定程度上实时量化地评估骨骼肌再灌注损伤的严重程度。

Objective:To observe the changes of iFlow parameters of ischemia reperfusion injury in rabbit hind limb skeletal muscle after acute ischemia using parametric digital subtraction angiography(DSA)color coding(syngo iFlow)and to explore the application in evaluating the ischemia-reperfusion injury of skeletal muscle.Methods:Thirty New Zealand white rabbits were randomly divided into five groups,under 3 h of right hind limb ischemia followed by 0,6,12,and 24 h of reperfusion(IR groups,six rabbits were used for each reperfusion interval),and six rabbits were served as Sham-operated control group.The rabbits in reperfusion groups accepted DSA scanning of double lower limbs and obtained the iFlow parameters(rPeak)by syngo iFlow software.Meanwhile,the serumal LDH,CK,MDA and SOD of the sample from jugular vein were measured,and the correlation between each iFlow parameter and serumal biochemical indicator was analyzed.Results:Compared with the control group,the serumal CK,LDH and MDA in IR groups increased(P<0.05),while the value of SOD decreased with the extension duration of reperfusion(P<0.05).Compared with the control group,the rPeak of IR groups decreased gradually with the prolongation of reperfusion(F=167.78,P<0.05).The coefficients to correlation between rPeak and LDH,CK,MDA,SOD respectively were-0.885,-0.908,-0.541 and 0.832(P<0.05).Conclusion:The correlation between iFlow parameters and serumal biochemical indicators are fairly good,and iFlow parameters can dynamically monitor the degree of ischemia reperfusion injury skeletal muscle.