反相高效液相色谱法测定复方壮药艾条中桂皮醛含量

Content Determination of Cinnamaldehyde in Moxa Sticks with Compound Zhuang Medicine by RP-HPLC

目的建立测定复方壮药艾条中桂皮醛含量的反相高效液相色谱法。方法色谱柱采用Agilent 5 TC-C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(35∶65),检测波长为290 nm,流速为1 m L/min,柱温为35℃。结果桂皮醛达到基线分离,进样量在0.028~0.336μg范围内与峰面积线性关系良好(r=0.999 9);平均加样回收率为102.39%,RSD为2.86%(n=6)。不同批号的复方壮药艾条中桂皮醛的含量为0.595~0.648 mg/g。结论该方法操作简单、快速、重复性好,可更好地控制复方壮药艾条的质量。

Objective To establish a RP-HPLC method to determine the content of cinnamicaldehyde in moxa sticks with compound Zhuang medicine.M ethods Agilent 5 TC-C18 column(250 mm x 4.6 mm,5μm)was adopted,the mobile phase was acetonitrile-water(35:65),the detection wavelength was 290 nm,the flow rate was 1 mL/min,and the column temperature was 35℃.Results The baseline separation of cinnamaldehyde was achieved.The linear range of cinnamaldehyde was 0.028-0.336μg(r=0.999 9).The average recovery was 102.39%and RSD was 2.86%(n=6).The average content of cinnamaldehyde was 0.595-0.6 48mg/g from different batches.Conclusion The method is simple,rapid and reproducible,which can be used for quality control of moxa sticks compound Zhuang medicine.