载阿仑膦酸钠的磷酸钙骨水泥修复兔股骨骨缺损的实验研究

Experimental study of alendronate-loaded calcium phosphate cement in the repair of femoral bone defects in rabbits

目的探究载阿仑膦酸钠(ALN)的磷酸钙骨水泥(CPC)修复兔股骨骨缺损的临床效果。方法选取36只健康成年新西兰大白兔,按随机数表法分为两组,各18只。于双侧股骨内侧髁处制作骨缺损动物模型,给予观察组ALN/CPC复合材料修复骨缺损,给予对照组CPC植入修复骨缺损。术后两组大白兔在相同的环境下饲养,于术后4、8、12周时分别处死6只兔子,取股骨髁处标本进行X线检查、骨密度测定,观察两组新骨形成情况。结果两组材料均未见明显的炎症组织反应,其组织相容性均良好,通过影像学检查、骨密度测定可发现:术后4周CPC柱和两端股骨界面清晰可辨,未见骨痂形成;8周界面模糊,可见少量骨痂生长;12周CPC柱与骨缺损交界处被大量骨痂包裹;术后4、8、12周观察组的骨痂形成速度和效果优于对照组。术后4、8、12周观察组的骨密度均高于对照组,差异有统计学意义(P<0.05)。结论载阿仑膦酸钠的磷酸钙骨水泥可促进兔股骨骨缺损的修复,增强股骨强度。

Objective To investigate the effect of alendronate-loaded calcium phosphate cement on repair of femoral bone defects in rabbits.Methods Thirty-six healthy adult New Zealand white rabbits were selected and divided into two groups according to the random number table.The animal model of bone defect was made at bilateral medial femoral condyle.ALN/CPC composite was used to repair the bone defect in the observation group.The control group was implanted with CPC to repair the bone defect.Six rabbits were sacrificed at the fourth,eighth and twelfth weeks after operation.The X-ray and bone mineral density of the femoral condyle were measured and the formation of new bone was observed.Results There was no obvious inflammatory tissue reaction between the two groups,and the histocompatibility was good.According to the imaging examination,the bone mineral density of the two groups showed that the CPC column and the femur interface at both ends were clearly discernible after 4 weeks,8 weeks after operation,the callus formation rate and the effect of the observation group were better than those of the control group at fourth,eighth and twelfth weeks after operation.The bone mineral density of the observation group at fourth,eighth and twelfth weeks after operation was significantly higher than that of the control group(P<0.05).Conclusion Calcium phosphate cement containing alendronate sodium can promote the repair of femoral bone defects in rabbits and enhance the strength of the femur.