肾衰1号对肾脏缺血再灌注大鼠KIM-1和NGAL蛋白表达的影响

Effect of Shenshuai No.1 on sensitive proteins KIM-1 and NGAL in kidney ischemia-reperfusion rats

[目的]探讨肾衰1号对肾脏缺血再灌注大鼠肾脏损伤敏感蛋白肾损伤因子-1(KIM-1)和中性粒细胞明胶酶相关脂质运载蛋白(NGAL)的影响。[方法]将SD大鼠随机分为5组:假手术组、模型组、尿毒清颗粒阳性药组、肾衰1号高剂量组和肾衰1号低剂量组。除假手术组外,其余各组均进行双侧肾结扎复灌,造成急性缺血再灌注损伤。分别连续给药1d、3d、7d,实验结束后,测定血清尿素氮(BUN)和血清肌酐(SCr),观察肾组织病理形态学,免疫印迹法测定肾脏组织中KIM-1和NGAL的表达。[结果]模型组大鼠BUN和SCr和肾组织中KIM-1和NGAL水平明显增高(P<0.001);与模型组比较,肾衰1号高低剂量组BUN、SCr均有明显降低(P<0.001;P<0.001,P<0.01),NGAL蛋白表达有明显下降(P<0.01)。HE病理染色显示肾衰1号能够减少细胞管型、肾小管上皮细胞坏死、组织出血及炎性反应的发生与发展,其中以肾衰1号高剂量效果最为明显。[结论]肾衰1号通过降低KIM-1和NGAL的表达发挥对肾脏的保护作用。

[Objective]To investigate the effect of Shenshuai No.1 on the sensitive proteins KIM-1 and NGAL in kidney ischemia-reperfusion rats.[Methods]SD rats were randomly divided into 5 groups:sham operation group,model group,Niaoduqing granule positive group,high dose group and low dose group of Shenshuai No.1.In addition to the sham operation group,all the other groups underwent bilateral renal ligation and reperfusion,which resulted in acute ischemia-reperfusion injury.Continuous medication was used for 1 day,3 days,7 days after the operation.Serum urea nitrogen(BUN)and creatinine(SCr)were detected,the renal pathological morphology was observed,KIM-1 and NGAL of kidney were detected by Western Blot.[Results]Serum urea nitrogen(BUN)and creatinine(SCr)levels and the level of NGAL and KIM-1 in renal tissue of model group rats increased significantly(P<0.001).Compared with the model group,the level of serum BUN and SCr of Shenshuai No.1 high dose group and low dose group were significantly reduced(P<0.001;P<0.001,P<0.01),the protein expression of NGAL decreased significantly(P<0.01).HE staining showed that Shenshuai No.1 can reduce the occurrence and development of renal tubular epithelial cells,tubular cell necrosis,bleeding and inflammatory reaction,the effect of Shenshuai No.1 high dose was more obvious.[Conclusion]Shenshuai No.1 can protect the kidney by reducing the expression of KIM-1 and NGAL.