萱草属种质遗传多样性分析及初级核心种质库的构建

Genetic diversity analysis of Hemerocallis and constructing the primary core collection

本试验在系统收集萱草属种质资源的基础上,以155份萱草属种质资源为材料,采用EST-SSR分子标记对其进行遗传多样性和聚类分析,基于分子标记聚类结果采用多次聚类优先取样策略构建萱草属初级核心种质库,并对初级核心种质库进行遗传多样性和聚类分析。结果表明:(1)43对引物对155份萱草属种质共扩增出396条多态片段,平均扩增9.21条,引物多态性信息含量(PIC)平均值为0.6064,不同材料间的遗传相似系数平均为0.8642,依据遗传相似系数及聚类树状图,将155份种质聚类分为"黄花菜"和"萱草"2大类群。(2)通过6次取样建立萱草属初级核心种质库,包含32份种质(4个种、1个变种、27个品种)。初级核心种质库为原始群体的20.65%,保留了291个(68.69%)多态性位点,其中‘黄花菜’类群种质8份,占‘黄花菜’类群资源总数的13.56%;‘萱草’类群种质24份,占‘萱草’类群资源总数的25.00%。(3)43对引物对32份核心种质共扩增出272条多态片段,平均扩增6.3256条,PIC值平均值0.6060,不同种质间的遗传相似系数平均为0.7940;32份材料聚类分为"黄花菜"和"萱草"2大类群。

155 Hemerocallis germplasm resources were used as materials based on systematic collection,and genetic diversity and cluster analysis were carried out using EST-SSR molecular markers.Based on the clustering result of molecular marker,the procedure of stepwise clustering preferred sampling strategy was used to construct the Hemerocallis primary core collection and then the genetic diversity and cluster analysis were carried out for the primary core collection.The results were as follows:(1)A total of 396 polymorphic fragments were amplified from 43 pairs primers on 155 lines of Hemerocallis,and with an average of 9.21 per marker.The average value of polymorphism information content(PIC)was 0.6064 and the genetic similarity coefficient was 0.8642.Based on the genetic similarity coefficient and cluster tree,155 lines were clustered into two groups:“the long yellow daylily”and“daylily”.(2)Hemerocallis primary core collection was established by 6 samplings,including 32 lines(4 species,1 variety,27 varieties).The primary core collection was 20.65%of the original population,with 291(68.69%)polymorphic loci retained,of which 8 were“the long yellow daylily”group,accounting for 13.56%of the total population of“the long yellow daylily”group.24 lines were“daylily”group,accounting for 25.00%of the total number of“daylily”group.(3)A total of 396 polymorphic fragments were amplified from 43 pairs of primers on 32 genotypes of the core collection of Hemerocallis,with an average of 6.3256 per marker.The average value of PIC was 0.6060 and the genetic similarity coefficient was 0.7940.33 lines of primary core collection were divided into two groups:“the long yellow daylily”and“daylily”.