人参蛋白质不同提取方法及含量的比较

Comparision on Different Extraction Methods and Contents of Panax ginseng Protein

[目的]优选人参蛋白质提取的最佳方法,并比较不同生长年份对人参蛋白质含量的影响。[方法]比较丙酮沉淀法、聚乙二醇6000沉淀法、聚乙二醇20000沉淀法、酚提取法、Trizol提取法5种人参蛋白质的提取方法,及不同生长年份的人参蛋白质,采用Bradford方法测定蛋白质含量,SDS-PAGE电泳鉴定蛋白。[结果]丙酮提取法得到的蛋白质纯度最高,电泳条带最清晰完整,且人参蛋白质的含量随生长年份增加而升高。[结论]丙酮沉淀法是一种较好的适用于人参蛋白质提取的方法,5年生人参蛋白质含量最高。

[Objective]The best method of extraction of ginseng protein was optimized,and the effect of different growth years on protein content was compared.[Method]The extraction method of five kinds of ginseng protein was studied by acetone precipitation method,polyethylene glycol 6000 precipitation method,polyethylene glycol 20000 precipitation method,phenol extraction method and Trizol extraction method,and different growth years of ginseng protein.The protein content was determined by Bradford method,SDS-PAGE identification of proteins.[Result]The purity of the protein was the highest in the acetone extraction method,and the electrophoretic bands were the most clear and complete,and the content of ginseng protein increased with the growth year.[Conclusion]Acetone precipitation method was a better method for the extraction of ginseng proteins.The protein content of ginseng was the highest in 5 years.