超声波辅助酶法提取油茶籽壳色素工艺研究

Optimization of Ultrasonic-assisted Enzymatic Extraction of Camellia oleifera Seed Shells Pigment

[目的]优化超声波辅助酶法提取油茶籽壳色素的最佳工艺。[方法]以油茶籽壳为原材料,采用超声波辅助酶法提取油茶籽壳色素,以响应面试验优化其提取条件。[结果]最佳提取条件:加酶量为0.8%,液固比20∶1(g∶mL),超声提取时间15 min,超声提取功率90 W,超声提取温度60℃。在此条件下测得的吸光度为2.765。酶辅助超声波法提取油茶籽壳色素较酶法和超声波提取法油茶籽壳色素吸光度提高了1.8、1.5倍。[结论]该研究优化了超声波辅助酶法提取油茶籽壳色素最佳工艺条件,为油茶籽壳色素的综合开发利用提供科学依据。

[Objective]To optimize ultrasonic-assisted enzymatic extraction of Camellia oleifera seed shells pigment.[Method]Using ultrasonic-assisted enzymatic method to extract the pigment from Camellia oleifera seed shell,optimization of the extraction conditions by single factor and response surface test were done.[Result]The optimum condition for extraction was that the enzyme dosage was 0.8%,the liquid solid ratio was 20∶1(g∶mL),the ultrasonic time of extraction was 15 min,the ultrasonic power of extraction was 90 W,the ultrasonic temperature of extraction was 60℃.The absorbance value of Camellia oleifera seed shell pigment measured under this circumstance was 2.765.The pigment absorbance of Camellia oleifera seed shell was improved 1.8 times and 1.5 times by enzyme-assisted ultrasonic method compared to enzymatic method and ultrasonic extraction method.[Conclusion]The study optimizes ultrasonic-assisted enzymatic extraction condition of Camellia oleifera seed shells pigment,and provides scientific basis for the further research and development of the natural pigment of Camellia olifera seed shell.