三七提取物抗谷氨酸损伤PC12细胞的活性筛选及成分分析

Viability screening and composition analysis of panaxnotoginseng extracts against glutamate-induced PC12 cells

目的研究三七提取物抗谷氨酸损伤PC12细胞的活性筛选,并分析活性部位的化学成分。方法以谷氨酸损伤PC12细胞为模型,实验分为对照组、模型组、石油醚提取物和超临界CO_2萃取物组(6.25、12.50、25、50、100、200μg/m L)。采用MTT比色法对三七石油醚提取和超临界CO_2萃取部位进行活性筛选,并利用气质联用技术(GC-MS)对三七石油醚提取物和超临界CO_2萃取物进行化学成分鉴定分析。结果与对照组比较,25 mmol/L谷氨酸作用PC12细胞24 h,细胞生长抑制率接近50%。不同浓度石油醚提取物组与模型组的细胞存活率差异有统计学意义(P<0.05),25、50、100μg/m L石油醚提取物组间的细胞存活率差异有统计学意义(P<0.05)。不同浓度超临界CO_2萃取物组与模型组的细胞存活率差异有统计学意义(P<0.05),25、50、100μg/m L超临界CO_2萃取物组间的细胞存活率差异有统计学意义(P<0.05)。50、100μg/m L超临界CO_2萃取物组与石油醚提取物组的细胞存活率差异有统计学意义(P<0.05)。经GC-MS技术分析得出,超临界CO_2萃取物中主要的脂溶性成分人参炔醇含量(13.09%)高于石油醚提取物(4.10%)。结论石油醚提取物与超临CO_2萃取物对PC12细胞均有保护作用,但超临界CO_2萃取物具有较好的细胞保护作用。

Objective To research activity screening and composition analysis of panaxnotoginseng extracts against glutamate-induced PC12 cells.Methods Glutamate-induced toxicity to PC12 cells was used to model cerebral ischemia injury.The experimental animals were divided into control group,model group,and 6.25,12.50,25,50,100,200 ug/mL drug groups.In addition,the activities of petroleum ether extraction and supercritical CO 2 extraction on PC12 cells were detectedby MTT,and the extracts determined andanalysed by GC-MS.Results Compared with control group,the cell growth inhibitory rate increased to nearly 50%for 25 mmol/L glutamate in 24 h.Hence,25 mmol/L glutamate and 24 h incubation time for glutamate model conditions were chosen in our assays.The difference in cell survival rate between petroleum ether extract group of different concentrations and model group was statistically significant(P<0.05).There was significant difference in cell survival rate between 25,50,100 ug/mL petroleum ether extract group(P<0.05).The difference in cell survival rate between supercritical CO 2 extract group of different concentrations and model group was statistically significant(P<0.05).There was significant difference in cell survival rate between 25,50,100 ug/mL supercritical CO 2 extract group(P<0.05).There was significant difference in cell survival rate between 50,100 ug/mL supercritical CO 2 extract group and petroleum ether extract group(P<0.05).In addition,the content of the panaxynol of supercritical CO 2 extract(13.09%)was remarkably higher than that of petroleum ether extract(4.10%)by GC-MS analysis.Conclusion Both petroleum ether extract and supercritical CO 2 extract have protective effects on PC12 cells.However,the protective action of supercritical CO 2 extract is significantly stronger than that of petroleum ether extract.