摘要
目的探讨沉默叉头框转录因子M1(Fox M1)对人甲状腺乳头状癌细胞株K1增殖、迁徙及侵袭的影响。方法用脂质体LipofectamineTM2000分别将Fox M1-siRNA及control siRNA转入人甲状腺癌K1细胞株中,作为转染组及无意义序列组,另取一株K1细胞添加PBS溶液作为空白对照组;免疫印迹试验检测Fox M1蛋白表达;MTT法检测各组细胞增殖能力;单层细胞划痕实验检测各组细胞迁徙能力;Transwell实验检测各组细胞侵袭能力。结果空白对照组、无意义序列组、转染组Fox M1蛋白相对表达量分别为0.96±0.01、0.95±0.01、0.27±0.03,转染组与空白对照组、无意义序列组比较,P均<0.05;空白对照组与无意义序列组比较,P>0.05。转染组细胞生长受到抑制,与无意义序列组、空白对照组比较,P均<0.05;无意义序列组与空白对照组比较,P>0.05。24 h后,空白对照组、无意义序列组、转染组划痕面积相对比值分别为0.83±0.01、0.82±0.01、0.93±0.01,转染组与空白对照组、无意义序列组比较,P均<0.05,空白对照组与无意义序列组比较,P>0.05。空白对照组、无意义序列组、转染组穿过上小室背面的细胞数分别为(92.40±3.05)、(85.40±5.13)、(37.20±3.96)个/视野,转染组与空白对照组、无意义序列组比较,P均<0.05,空白对照组与无意义序列组比较,P>0.05。结论沉默Fox M1可抑制人甲状腺乳头状癌K1细胞增殖,降低细胞迁徙及侵袭能力。
Objective To investigate the effects of silencing forkhead box transcription factor M1(FoxM1)on the proliferation,migration,and invasion of human papillary thyroid carcinoma cell line K1.Methods Using liposome Lipofectamine TM 2000 to separately transfect the FoxM1-siRNA and control-siRNA into the human thyroid carcinoma cell line K1,as the transfection group and meaningless sequence group;another cell line K1 was added with PBS solution as the blank control group.The expression of FoxM1 protein was detected by Western blotting;the cell proliferation ability was determined by MTT assay;the cell migration in each group was detected by single cell scratch test;the cell invasion ability in each group was detected by Transwell invasion test.Results The relative expression of FoxM1 protein in the blank control group,the meaningless sequence group,and the transfection group were 0.96±0.01,0.95±0.01 and 0.27±0.03;significant difference was found between the transfection group and the blank control group and the meaningless sequence group(all P<0.05),but no significant difference was found between the blank control group and the meaningless sequence group(P>0.05).The cell growth of the transfection group was inhibited,and significant difference was found between the meaningless sequence group and the blank control group(P<0.05),but no significant difference was found between the blank control group and the meaningless sequence group(P>0.05).At 24 h,the relative ratios of scratch area in the blank control group,meaningless sequence group,and transfection group were 0.83±0.01,0.82±0.01,and 0.93±0.01,respectively;the number of cells passing through the back of the upper chamber in the blank control group,the meaningless sequence group,and the transfection group were(92.40±3.05),(85.40±5.13),and(37.20±3.96)/field;significant difference was found between the transfection group and the blank control group and the meaningless sequence group(all P<0.05),but no significant difference was found between the blank control group and the meaningless sequence group(P>0.05).Conclusion Silencing FoxM1 inhibits the proliferation of human papillary thyroid carcinoma K1 cells,and reduces the cell invasion and migration.
作者
刘千玉
张国志
田炜
詹琪琪
陈建立
陈俊卯
曹文斌
LIU Qianyu;ZHANG Guozhi;TIAN Wei;ZHAN Qiqi;CHEN Jianli;CHEN Junmao;CAO Wenbin(Affiliated Hospital of North China University of Science and Technology,Tangshan 063000,China)
出处
《山东医药》
CAS
2018年第9期17-20,共4页
Shandong Medical Journal
基金
河北省科学技术情报研究院资助项目(20150523)
关键词
甲状腺乳头状癌
叉头框转录因子M1
细胞增殖
细胞迁徙
细胞侵袭
thyroid papillary carcinoma
forked frame transcription factor M1
cell proliferation
cell migration
cell invasion