脑梗死急性期大鼠胃黏膜损伤情况及意义

Dynamic changes of gastric mucosal injury in acute stage of cerebral infarction

目的探讨脑梗死急性期大鼠胃黏膜损伤情况及意义。方法选择雄性Wistar大鼠56只,随机分为对照组8只、假手术组24只、脑梗死组24只。脑梗死组采用线栓法建立大脑中动脉梗塞模型;假手术组只结扎血管,不插入尼龙鱼线;对照组不予处理。假手术组和脑梗死组分别于造模1、4、7天各取8只,对照组任意时间检测血清胃泌素(GAS)水平;采血后处死,取胃组织行HE染色,显微镜下观察,并进行胃黏膜病理评分。结果脑梗死组各时间血清GAS水平较对照组及假手术组均升高(P均<0.05),对照组与假手术组各时间血清GAS水平比较P均>0.05;随造模时间延长,脑梗死组血清GAS水平逐渐下降(P均<0.05)。对照组和假手术组各时间胃黏膜大体形态无明显变化,胃黏膜病理评分均为0分。脑梗死组各时间胃黏膜损伤程度重于对照组和假手术组,胃黏膜病理评分均高于对照组和假手术组(P均<0.05),且造模4天时胃黏膜病理评分最高。结论脑梗死急性期大鼠可见胃黏膜损伤,其黏膜损伤与脑梗死本身有关,高胃泌素血症可能不是胃黏膜损伤的主要原因。

Objective To explore the dynamic changes of gastric mucosal injury in acute stage of cerebral infarction.Methods Fifty male Wistar rats were selected and randomly divided into the control group(n=8),sham operation group(n=24),and cerebral infarction group(n=8).The middle cerebral artery occlusion model in the cerebral infarction group was established by the line embolus.The blood vessels of the rats in the sham operation group were only ligated without the nylon fish line insertion.The control group was not treated.In the sham operation group and the cerebral infarction group,8 rats were taken on day 1,4 and 7,respectively.The serum level of gastrin(GAS)was detected at any time in the control group.After the blood was collected,the gastric tissues were stained by HE staining.The pathological grading of gastric mucosa was observed and photographed under microscope.Results The serum GAS level of rats in the cerebral infarction group at each time was significantly higher than that in the control group and sham operation group(all P<0.05),but there was no difference in serum GAS content between the control group and sham operation group(P>0.05).The level of serum GAS in the cerebral infarction group decreased with time(P<0.05).In the control group and the sham operation group,the gross morphology of the gastric mucosa did not significantly change,and the pathological score of the gastric mucosa was 0.The degree of gastric mucosal injury in cerebral infarction group was worse than that of the control group and the sham operation group,but the pathological scores of gastric mucosa were higher than those in the control group and sham operation group(all P<0.05),and the highest pathological score of gastric mucosa was on the 4 th day of modeling.Conclusion In the acute phase of acute cerebral infarction,gastric mucosal injury can be seen in rats,and its mucosal injury is related to cerebral infarction itself.Hypergastrinemia may not be the main factor of gastric mucosal injury.