赣州市活禽市场禽流感病毒污染状况

Study on contamination status of avian influenza virus in live poultry markets in Ganzhou city

目的通过分析活禽市场禽流感病毒污染状况,为疫情防控和预警提供依据。方法 2015年1月、8月和2017年1月,3次采集赣州市各地活禽市场不同类型环境标本,采用RT-PCR方法检测标本中禽流感病毒核酸(FluA),对阳性标本进行H5、H7及H9亚型检测。结果共采集787份标本,FluA阳性467份,阳性率59.34%,H5、H7、H9亚型阳性率分别为16.01%、19.44%和40.79%;混合感染率为20.84%,占FluA阳性标本的35.12%。3次监测中FluA、H7、H9亚型阳性率差异均有统计学意义(!2值为76.31、98.91、122.63,P值均<0.01);其中冬季H7亚型阳性率高于夏季,夏季H9亚型阳性率高于冬季,差异均有统计学意义(!2值为61.71、35.38,P值均<0.01)。结论赣州市活禽市场禽流感病毒污染较严重,以H9亚型为主,病毒混合感染程度较高。H7亚型阳性率为冬季高于夏季,而H9亚型禽流感病毒则相反。

Objective To analyze the avian influenza virus contamination status in live poultry markets;to provide evidence for the prevention and control of human infection with avian influenza virus.Methods Different types of environmental samples were collected from live poultry markets in Ganzhou city at Jan 2015,Aug 2015 and Jan 2017,which were subjected to nucleic acid analysis for avian influenza virus(AIV FluA)by RT-PCR.The positive samples were analyzed for H5,H7,H9 subtype nucleic acid.Results A total of 787 environmental samples were collected,467 samples were positive for FluA,AIV FluA positive rate was 59.34%;the positive rates of H5,H7,and H9 were 16.01%,19.44%,and 40.79%,respectively.The mixed infection rate was 20.84%,accounting for 35.12%of FluA positive specimens.Among three surveillane time points,the positive rates of AIV FluA,H7,H9 subtype were different,with statistical differences(0x0E?SymbolcA@0x0F 2=76.31,98.91,122.63,all P<0.01).The positive rate of subtype H7 was significantly higher in winter than that in summer,while the positive rate of subtype H9 was higher in summer than that in winter,with statistical difference(0x0E?SymbolcA@0x0F 2=61.71,35.38,all P<0.01).Conclusion The AIV contamination status in live poultry markets was serious in Ganzhou;the subtype H9 was the dominant epidemic strain.There mixed infection rate of AIV was high.The positive rates of subtype H7 was higher in winter season than in summer,while subtype H9 showed higher positive rate in summer.