摘要
为降低由人工授精诱导的猪子宫局部免疫反应,从而提高其人工授精效率,在含有10%血清的培养液中添加不同浓度的肝素(0、100、500、1 000μg/mL),检测猪嗜中性粒细胞(PMNs)对猪精子的吞噬性和趋化性。结果表明,100~1 000μg/mL的肝素可显著抑制猪PMNs对精子的吞噬性(P<0.01)和趋化性(P<0.05)。提示肝素可作为猪精液稀释液的保护成分,降低由人工授精所导致的子宫炎症反应,从而提高猪的人工授精受胎率。
In order to suppress the local immune response in swine uterus induced by artificial insemination(AI)and to improve the AI application efficiency,the regulative effect of different dosages of heparin(0,100,500,1 000μg/mL)on changes of chemotaxis and phagocytosis of swine polymorphonuclear neutrophils(PMNs)against sperm were observed with the presence of 10%serum in an in vitro culture system.The results showed that the chemotaxis and phagocytosis of swine PMNs against sperm was found to be significantly inhibited by heparin at the dosage of 100-1 000μg/mL(P<0.05 or P<0.01).It is demonstrated that heparin can effectively suppress the immune response in swine uterus induced by AI,and it is prospected to be used as the protectant of swine semen diluents to improve the conception rate of the artificially inseminated female swine.
作者
李井春
李琦
韩淑敏
张帆
孙思怡
李雁冰
魏国生
LI Jing-chun;LI Qi;HAN Shu-min;ZHANG Fan;SUN Si-yi;LI Yan-bing;WEI Guo-sheng(College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China)
出处
《畜牧与饲料科学》
2018年第3期7-9,共3页
Animal Husbandry and Feed Science
基金
大庆市指导性科技计划项目(zd-2016-087)
国家自然科学基金项目(31402136)
黑龙江省自然科学基金面上项目(C2017053)
黑龙江省农垦总局科研计划项目(HNK135-04-06
HNK135-04-02-03)
黑龙江八一农垦大学博士启动基金项目(XYB-2016-04
B2016-06)
黑龙江八一农垦大学创新人才项目(CXRC2017004
CXRC2017007)
黑龙江八一农垦大学研究生创新科研项目(YJSCX2017-Y16)
关键词
肝素
猪嗜中性粒细胞
趋化性
吞噬性
heparin
swine polymorphonuclear neutrophils
chemotaxis
phagocytosis
