基于微流控芯片SERS生物传感器的发展与应用

Development and application of SERS biosensor based on microfluidic chip

表面增强拉曼光谱(surface enhanced Raman spectroscopy,SERS)作为一种新兴的、具有超高灵敏度无标记指纹的高级痕量分析技术,与微流控芯片相结合用于光流体检测可以实现快速、无损伤检测分析。稳定、重现、高强度、高灵敏度的SERS信号是获取有效生物传感信息的首要条件。大量研究表明,可控性金属纳米粒子聚集体或纳米颗粒阵列对产生上述SERS信号至关重要。本文综述了利用电场、磁场形成可控性金属纳米粒子聚集体、固体SERS活性基底对高灵敏度SERS信号的增强和重现作用,固态SERS活性基底对其体表面积的增加作用,以及SERS生物传感器在临床免疫测定中的应用等方面的研究进展,为进一步深入研究贵金属纳米结构对SERS信号的增强、重现机制,特别是SERS生物传感器的应用提供参考,同时也为通过优化设计固态SERS活性基底进而实现便携、智能的SERS生物传感器系统提供一定的科学依据。

Surface enhance Raman spectroscopy(SER S)as an emerging advanced trace analysis technique with ultra-sensitive and fingerprint-free features,achieves fast and non-invasive detection combined with microfluidic chip in optical fluid detection.SERS signals with the features of stability,reproduction,highintensity and high-sensitivity are the prerequisites for obtaining valid bio-sensing information.A number of investigations demonstrate that noble metal nanoparticle aggregates of controllability or nanoparticle arrays are crucial for generating the above SERS signals.The research progress of certain aspects is summarized in this article,which includes the enhancement and reappearance of high-sensitivity SERS signals by using metal nanoparticle aggregates of controllability and solid SERS active substrate formed by utilizing electric and magnetic fields,the increasing effect of solid SERS substrate on body surface area,and the application of SERS biosensor in clinical immunoassay.This review provides references to further study on the mechanism of reproduction and enhancement of SERS signal by noble metal nanoparticles,especially the application of SERS bio-sensor,and offers scientific evidence for optimize design on solid SERS active substrates to achieve portable and intelligent SERS bio-sensor system.