EGFR在胃癌组织中的表达及其与预后的关系

Expression of EGFR in Gastric Cancer Tissue and Its Relationship with Prognosis

目的检测胃癌组织和正常胃组织中的人类表皮因子受体(EGFR)表达水平,分析EGFR与胃癌患者临床病理特征及预后的关系。方法收集该院和市中心医院2012年1月—2015年12月间的80例实施胃癌根治术的患者作为研究对象,并随访存活情况;选择同期20例正常胃组织作为对照;采用En Vision二步法检测EGFR表达水平,观察EGFR表达与正常胃组织和胃癌组织的关系。结果 EGFR在胃癌组织中和正常胃组织中的阳性表达差异有统计学意义(正常胃组织33/80,41.25%vs胃癌组织1/20,5.00%,P<0.01),胃癌组织内EGFR阳性表达与肿瘤的浸润深度(T1、T2期:3/18,16.67%vs T3、T4期:30/62,48.39%,P<0.05)、分化程度(高中分化9/36,25.00%vs低分化、印戒细胞癌24/44,54.55%,P<0.05)、淋巴结转移(无转移3/20,15.00%vs有转移30/60,50.00%,P<0.05)及TNM分期(Ⅰ、Ⅱ期6/27,22.22%vsⅢ、Ⅳ期27/53,50.94%,P<0.05)关系密切。EGFR阳性表达与胃癌患者生存时间显著相关(阳性患者MST为18.32个月vs阴性患者MST为34.17个月,P<0.01),EGFR是胃癌患者独立的预后因素(P<0.05)。结论 EGFR在胃癌组织中和正常人群胃组织中的表达状况存在显著性差异,并且与胃癌患者的肿瘤分化程度、浸润深度、淋巴结转移、TNM分期关系密切;多因素分析EGFR是独立的预后因素,但在胃癌中EGFR可能并非主要驱动基因。

Objective The expression levels of human epidermal receptor(EGFR)in gastric cancer tissues and normal gastric tissues were detected,the relationship between EGFR and clinicopathological features and prognosis of patients with gastric cancer were analyzed.Methods 80 patients with radical gastric cancer were selected as the research objects from January 2012 to December 2015 in this hospital and the central hospital,and survival circumstance were followed up;20 cases of normal gastric tissues were selected as the control group at the same period;the expression of EGFR was detected by EnVision two-step method,and the relationship between EGFR expression and normal gastric tissue and gastric cancer was observed.Results The positive expression of EGFR in gastric cancer tissue and normal gastric tissue was significantly different(normal gastric tissue 33/80,41.25%vs Gastric cancer tissue 1/20,5.00%,P<0.01);the positive expression of EGFR in gastric cancer was closely related to the depth of invasion(T1,T2 3/18,16.67%vs T3,T4 30/62,48.39%,P<0.05),differentiation(high and medium differentiation 9/36,25.00%vs poorly differentiated and signet ring cell carcinoma 24/44,54.55%,P<0.05),lymph node metastasis(no metastasis 3/20,15.00%vs metastasis 30/60,50.00%,P<0.05)and TNM staging(ⅠⅡstaging 6/27,22.22%vsⅢⅣstaging 27/53,50.94%,P<0.05),The positive expression of EGFR was significantly correlated with the survival time of gastric cancer patients(the median survival time of the positive patients was 18.32 months vs the median survival time of the negative patients was 34.17 months,P<0.01),EGFR was an independent prognostic factor for gastric cancer patients(P<0.05).Conclusion The expression of EGFR in gastric carcinoma and normal gastric tissue was significantly different,and it was closely related to the degree of tumor differentiation,depth of invasion,lymph node metastasis and TNM staging in gastric cancer patients and multivariate analysis showed that EGFR was an independent prognostic factor(P<0.01).However,EGFR may not be the main driving gene in gastric cancer.