接触性检材的DNA自动化提取

Forensic-purposed Automatic Extraction of DNA from Contact Samples

目的建立使用King Fisher磁珠纯化仪结合博坤超微量磁珠法提取试剂盒自动化提取接触性检材DNA的方法。方法进行不同起始量(0.1、0.15、0.2、0.25ng)的标准DNA回收、单枚指纹DNA提取、案件接触性检材检验三种实验。结果 10例0.1ng样本除2例外,其他浓度的标准DNA均检出完整分型。单枚指纹样本检出分型与被取样者分型比对,有效DNA检出率为57.2%。870例案件接触性检材检验结果,有效DNA检出率为71.5%。结论博坤超微量磁珠法提取试剂盒结合King Fisher磁珠纯化仪提取法医案件中常见生物样本的DNA,其产率和质量均能满足STR分型需要。

Objective To establish a method of forensic-purposed automatic DNA extraction from contact samples by KingFisher system combined with high-quality DNA extraction kit(BoKun).Methods Standard DNA was yielded from its different initial quantity(0.1ng,0.15ng,0.2ng,0.25ng),and DNA extraction was also individually conducted from either the single fingerprint deposits or the contact samples of forensic casework.Results Except 2 out of the 10 standard samples containing 0.1ng DNA,all the other concentrations of DNA got their complete STR genotypes.Compared with their contributors,the single fingerprint deposits achieved the effective DNA detection rate of 57.2%.For the tested 870 contact samples of forensic casework,the effective DNA detection rate was 71.5%.Conclusion High-quality DNA extraction kit,combined with KingFisher system,can be applied in the forensic-purposed DNA extraction from contact samples,yielding the DNA product suitable for STR typing with proper quantity and quality.