WZM5-EPS菌剂对土壤水肥保持及棉花幼苗生长的影响

Effect of WZM5-EPS agents on water and fertilizer conversation of soil, and the growth of cotton seedlings

WZM5是从内蒙古翁牛特沙漠土样中筛选得到的一株胞外多糖合成菌,形态学和16S rDNA序列的分子鉴定结果表明,WZM5属Massilia属,与Massilia plicata的同源性最高,为98.5%。菌株WZM5发酵液为高黏度的触变性流体,具有良好的假塑性,将其制成WZM5-EPS菌剂,分别喷施在不同质地土壤表面,具有明显的保水保肥效果,黏土、壤土和砂土的持水能力分别比对照增加了49.2%、83.9%和42.3%,砂土中铵态氮、磷酸根离子和钾离子的淋溶损失分别减少了36.1%、96.5%和36.3%。盆栽试验表明,WZM5-EPS菌剂不会影响棉花种子的萌发和幼苗出土,可促进棉花幼苗的生长。

Strain WZM5,extracellular polysaccharide synthesis-bacteria,was isolated from soil of Ongniod desert,Inner Mongolia.Morphology and phylogenetic analysis based on 16S rDNA gene sequences showed that strain WZM5 belongs to the genus of Massilia,exhibiting the highest sequence similarity with Massilia plicata(98.5%).The fermentation broth of WZM5 belongs to thixotropic fluid,with high viscosity and excellent pseudoplastic.When the microbial agent of WZM5 was sprinkled on the surface of soil,the ability of soil water conservation and fertilizer conservation increased.The results showed that the ability of water retention in clay,loam and sand increased 49.2%,83.9%and 42.3%,respectively,compared with the control.After leaching experiment,ammonium nitrogen leaching loss reduced 36.1%,phosphate fertilizer leaching loss reduced 96.5%,and potassic fertilizer leaching loss reduced 36.3%in sand.The results of pot experiment showed that cotton seed germination and seedling unearthed was not affected by WZM5-EPS microbial agent,but the growth of cotton seedling was promoted significantly.