当归产区根际土壤近红外漫反射光谱指纹图谱研究

Study on Near Infrared Diffuse Reflectance Spectrum Fingerprints of Rhizosphere Soil from Angelica Sinensis

以积分球漫反射方式采集11个当归产区共147批根际土壤样品的NIRDRS,解析特征吸收峰,采用"相关系数法"对NIRDRS指纹图谱进行相似度分析,建立正交偏最小二乘-判别分析(OPLS-DA)模型探讨其与土壤类型及产区划分的关系。结果表明8个主产县根际土壤的NIRDRS指纹图谱、一阶导数光谱及二阶导数光谱均较为相似,但临洮县、临潭县及康乐县等3个产区与其他产地(尤其是岷县)差异明显;基于OPLS-DA模型能够将各产区根际土壤的NIRDRS指纹图谱进行明确区分,其差异的主要波数范围为7352.5~6935.9 cm-1、5819.9~5760.4 cm-1、4927.1~4609.7 cm-1、4411.3~4485.7 cm-1及4307.1~4188.1 cm-1。当归产区根际土壤具有较为明显的地域性分布特征,根际土壤的NIRDRS指纹图谱与土壤类型密切相关。

Near infrared diffuse reflectance spectrum(NIRDRS)fingerprints of 147 batches of Rhizosphere soil from 11 areas produced Angelica sinensis of Gansu Province were collected by integral sphere diffuse reflection,and the characteristic absorption peaks of NIRDRS fingerprints were analyzed.The similarity analysis of NIRDRS fingerprints were carried out by"correlation coefficient method",and orthogonal partial least squares-discriminant analysis(OPLS-DA)model was established to study the relationship between soil type and area division.The NIRDRS fingerprints,first derivative spectra and second derivative spectra of Rhizosphere soil in 8 main producing counties were similar,but three producing areas such as Lintao County,Lintan County and Kangle County were obviously different with other producing areas(especially Min County).The NIRDRS fingerprints of the Rhizosphere soils in each producing area can be clearly distinguished based on the OPLS-DA model,and main wave numbers range of the differences existed 7352.5~6935.9 cm-1,5819.9~5760.4 cm-1,4927.1~4609.7 cm-1,4411.3~4485.7 cm-1,and 4307.1~4188.1 cm-1.Rhizosphere soil from Angelica sinensis has a distinct regional distribution,and the NIRDRS fingerprints of Rhizosphere soil was closely related to soil types.