不同叶色紫苏花青素含量与成分研究

The Contents and Constituents of Anthocyanidins from Different Perilla Leaves

选取不同叶色紫苏资源,统一田间栽培管理,待紫苏成熟期收获紫苏叶,并对紫苏叶中花青素含量进行分析。紫苏花青素传统的提取方法中含有大量的副产物如糖、有机酸和蛋白质等副产物,这些杂质可能加速花青素的降解。本研究比较不同提取介质的提取能力及选择性,其中乙醇-酸化水(50%,v/v)提取花青素的含量最高(4.7%)。采用不同的吸附剂进行吸附纯化,实验表明XAD-7HP吸附树脂表现出较好的吸附能力和解吸能力,利用LCMS对花青素苷成分进行分析,花青素苷中丙二酸单酰基紫苏宁含量最高。

With perilla as raw material,the anthocyanins in the leaves of different perilla varieties were studied.Perilla leaves were harvested at mature stage and analysis of anthocyanin content in the leaves of perilla varieties.Conventional extraction methods of anthocyanin from plant material are nonselective and yield pigment solutions with large amounts of byproducts such as sugars,organic acids and proteins.Some of these impurities may accelerate anthocyanin degradation.Different extracting media were used and the mixture of ethanol and acidified(50%,v/v)water resulted in maximum anthocyanin content(4.7%).In order to obtain anthocyanins in a purified form,adsorption was carried out with different adsorbents.Among these,nonionic acrylic ester adsorbent,namely Amberlite XAD-7HP showed the highest adsorption capacity and desorption ratio.The main components of anthocyanin were characterized by LCMS.The main components were malonylshisonin.