同位素稀释质谱法在临床参考系统中的应用

Applications of Isotope Dilution Mass Spectrometry in Clinical Reference Measurement System

测量结果溯源是实现医学实验室标准化最重要的策略,旨在保证不同实验室、不同测量方法、不同型号、不同仪器厂家检测结果的准确可比。参考测量程序用于评价其他测量程序获得的同类被测量值的正确度。同位素稀释质谱法(isotope dilution mass spectrometry,IDMS)是具有高计量学特性的基准方法。本文主要对同位素稀释质谱法在临床参考系统中的应用进行综述,介绍基本原理、定量方式及其不确定度,并结合国际检验医学溯源联合会(joint committee for traceability in laboratory medicine,JCTLM)参考系统数据库,分析同位素稀释质谱法在临床参考系统的应用及最新进展,为临床参考系统、同位素应用以及计量相关工作者提供参考。

Traceability is essential for standardization and harmonization of clinical analytical results.The measurement result can be comparable to a reference through a documented unbroken chain of calibrations each contribution to the measurement uncertainty.Reference measurement procedure defined as providing measurement results fit for intended use in assessing measurement trueness of measured quantity values obtained from other measurement procedures for quantities of the same kind.Isotope Dilution Mass Spectrometry is a primary standard acknowledged as having the highest metrological qualities and whose value is accepted without reference to other standards.The review focused on the applications of IDMS in clinical reference measurement system including its principle,quantitative approaches and its related uncertainty.The updated status of JCTLM database was also commented in this review,which is helpful for those related researchers in clinical measurement,isotope application as well as metrological fields.