基于EST-SSR及SRAP标记构建苦荬菜品种(系)DNA指纹图谱

DNA fingerprinting of Ixeris polycephala varieties based on EST-SSR and SRAP markers

苦荬菜是优良的一年生菊科青饲牧草,为构建苦荬菜品种(系)DNA指纹图谱,筛选出24对EST-SSR引物、32对SRAP引物组合构建指纹图谱。24对EST-SSR多态性引物对收集的14份苦荬菜品种(系)共扩增出270个清晰条带,多态性条带223个,PIC均值0.834,基因多样性指数变幅为0.2464~0.4288,Shannon指数变幅为0.3597~0.6148,可区分品种3~14个;32对SRAP引物检测到多态性条带367个,PIC均值0.826,基因多样性指数变幅为0.2006~0.3898,Shannon指数为0.3167~0.5716,可区分品种2~12个。7个引物在9个品种(系)上能扩增出特征谱带,综合各项遗传多样性指标筛选出IpSSR102、IpSSR80、IpSSR91、Me10+Em5、Me9+Em17,共5个核心引物的40个多态性条带构建苦荬菜品种(系)DNA指纹图谱,每个品种(系)都有一个特异分子身份指纹编码。

Ixeris polycephala is a member of the Asteraceae family with an annual growth habit and excellent forage quality.In order to construct DNA fingerprinting of 14 I.polycephala varieties,24 EST-SSR primers and 32 SRAP primers were screened for clear bands and high polymorphic imformation.A total of 270 bands including 223 polymorphic bands were amplified from the 24 EST-SSR primers,and the polymorphism information content values were up to 0.834.The Nei’s gene diversity(H)values ranged from 0.2464 to 0.4288,and values for Shannon’s information index(I)ranged from 0.3597 to 0.6148,while the number of distinguished varieties ranged from 3 to 14.For the 32 SRAP primers,367 polymorphic bands were detected,and the average polymorphism information content was 0.826.The Nei’s gene diversity(H)values ranged from 0.2006 to 0.3898,and values for Shannon’s information index(I)ranged from 0.3167 to 0.5716,while the number of distinguished varieties ranged from 2 to 12.Nine varieties produced unique bands from 7 primers.With more detailed consideration of each genetic diversity index,a total of 40 polymorphic bands were obtained from 5 core primers(IpSSR102,IpSSR80,IpSSR91,Me10+Em5,Me9+Em17),and for DNA fingerprinting of the 14 I.polycephala varieties each variety had a specific molecular or DNA fingerprint.