甜樱桃PacAMR1基因的克隆及表达分析

Cloning and expression analysis of PacAMR1 gene in sweet cherry

以甜樱桃果实为材料,利用反转录聚合酶链式反应(RT-PCR)技术,获得了参与调控甜樱桃As A生物合成的关键基因——Pac AMR1。序列同源性分析发现,该基因编码的氨基酸序列与其他植物AMR1蛋白具有较高的同源性。实时荧光定量RT-PCR的结果表明,随着果实发育成熟,Pac AMR1基因的表达量逐渐上升,而果实中的As A含量则是在果实发育早期较高。Pac AMR1基因的表达量在幼叶中最低,而在老叶中表达量最高;但相反的是,As A含量在幼叶中最高,而在老叶中最低。这些结果表明,Pac AMR1基因的表达可能参与了As A生物合成的负调控。

Ascorbic acid(AsA)biosynthesis in plants occurs through a complex network.Regulation about this network is largely uncharacterized.PacAMR1,a gene that regulates pathway on AsA biosynthesis was identified from sweet cherry by reverse transcription-polymerase chain reaction(RT-PCR).The results of homology analysis showed that PacAMR1 protein shares high sequence similarity with that of other plants.Real-time PCR analysis showed that the relative expression level of PacAMR 1 increased gradually with the development of fruit,however the AsA content was higher in early phage of fruit development.As leaves aged,PacAMR 1transcripts accumulated with a concomitant increase.In contrast,the AsA content was highest in young leaves and lowest in old leaves.These results indicated that PacAMR1 appears to play an important role in modulating AsA biosynthesis in sweet cherry by negative regulating.